文献类型：期刊文献

英文题名：Zoonotic Cryptosporidium Species and Enterocytozoon bieneusi Genotypes in HIV-Positive Patients on Antiretroviral Therapy

作者：Wang, Lin[1];Zhang, Hongwei[2];Zhao, Xudong[2];Zhang, Longxian[3];Zhang, Guoqing[4];Guo, Meijin[1];Liu, Lili[1];Feng, Yaoyu[1];Xiao, Lihua[4]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Sch Resources & Environm Engn, Shanghai 200237, Peoples R China;[2]Henan Ctr Dis Control & Prevent, Inst Parasite Dis Prevent & Control, Zhengzhou, Peoples R China;[3]Henan Agr Univ, Coll Anim Sci & Vet Med, Zhengzhou, Peoples R China;[4]Ctr Dis Control & Prevent, Atlanta, GA USA

年份：2013

卷号：51

期号：2

起止页码：557

外文期刊名：JOURNAL OF CLINICAL MICROBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000314108000025)】；

基金：This work was supported by the National Natural Science Foundation of China (grants 31110103901, 31229005, and 41001316); the National Basic Research Program of China (973 Project) (2011CB200903); Project of Medical Science and Technique of Henan, China (No. 201003142); Special Funding of the Henan Health Science and Technology Innovation Talent Project; Fundamental Research Funds for the Central Universities, China; and Open Funding Projects of the State Key Laboratory of Veterinary Etiological Biology at the Lanzhou Veterinary Research Institute and State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology.

语种：英文

摘要：Molecular diagnostic tools have been used increasingly in the characterization of the transmission of cryptosporidiosis and microsporidiosis in developing countries. However, few studies have examined the distribution of Cryptosporidium species and Enterocytozoon bieneusi genotypes in AIDS patients receiving antiretroviral therapy. In the present study, 683 HIV-positive patients in the National Free Antiretroviral Therapy Program in China and 683 matched HIV-negative controls were enrolled. Cryptosporidium species and subtypes and Enterocytozoon bieneusi genotypes were detected and differentiated by PCR and DNA sequencing. The infection rates were 1.5% and 0.15% for Cryptosporidium and 5.7% and 4.2% for E. bieneusi in HIV-positive and HIV-negative participants, respectively. The majority (8/11) of Cryptosporidium cases were infections by zoonotic species, including Cryptosporidium meleagridis (5), Cryptosporidium parvum (2), and Cryptosporidium suis (1). Prevalent E. bieneusi genotypes detected, including EbpC (39), D (12), and type IV (7), were also potentially zoonotic. The common occurrence of EbpC was a feature of E. bieneusi transmission not seen in other areas. Contact with animals was a risk factor for both cryptosporidiosis and microsporidiosis. The results suggest that zoonotic transmission was significant in the epidemiology of both diseases in rural AIDS patients in China.