文献类型：专利

英文题名：New S-adenosyl homocysteine nucleotidase polypeptide separated from Serratia marcescens, useful for separating coding gene

作者：QIU Y;SHEN Y;SUN J;WEI D;YANG Y;ZHANG L;ZHOU W;ZHU H

机构：[1]UNIV EAST CHINA SCI & TECHNOLOGY

申请号：CN101429500-A

申请日：2007-11-09

公开日：2009-05-13

语种：英文

收录：DERWENT

摘要：NOVELTY - New S-adenosyl homocysteine nucleotidase polypeptide separated from Serratia marcescens, where it is selected from: (i) a polypeptide containing fully defined 233 amino acids (SEQ ID NO: 2), given in the specification; and (ii) a polypeptide obtained by substituting, deleting or adding one or several amino acid residues with hydrolysis function on S-adenosyl homocysteine. USE - The S-adenosyl homocysteine nucleotidase polypeptide, vector, genetically engineered host cell, and methods are useful for separating coding gene of the S-adenosyl homocysteine nucleotidase polypeptide from S. marcescens (all claimed). DETAILED DESCRIPTION - INDEPENDENT CLAIMS are: (1) a separated polynucleotide, including an amino acid sequence that is selected from: (i) a polynucleotide coding the polypeptide above; and (ii) a polynucleotide complementary with the polynucleotide in (i); (2) a vector, comprising the polynucleotide; (3) a genetically engineered host cell, comprising the vector; (4) a method for preparing the polypeptide; and (5) a method for separating coding gene of the S-adenosyl homocysteine nucleotidase polypeptide from S. marcescens, comprising: (a) digesting S. marcescens genome DNA with restrictive internal cutting enzyme Hind III, separating 3-5 kb of DNA segments from the digested product and making the DNA segments cyclize and interconnect to form a self-cyclized molecule; (b) taking the self-cyclized molecule obtained in (a) as template, carrying out PCR amplification with the primers shown in cagtgttccagtagcagggtgg (SEQ ID NO: 5) and tgtcggaagaagtgcgctacc (SEQ ID NO: 6), collecting the amplified product with molecular weight at 652-752 bp; and (c) sequencing and verifying the amplified product obtained in (b) to obtain gene including a complete reading frame as the coding gene of the S-adenosyl homocysteine nucleotidase.