文献类型：期刊文献

英文题名：TGF-β1 fused to elastin-like polypeptides nanoparticles with kartogenin loading for mesenchymal stem cell directed cartilage differentiation and promoting in vivo repair

作者：Fu, Yunhui[1];Li, Yuxi[1];Wu, Chenlu[1];Chen, Yiying[1];Zhang, Chen[1];Tong, Xikui[1];Wang, Yangmin[1];Shen, Yue[1];Zheng, Wenyun[2];Ma, Xingyuan[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Shanghai Key Lab New Drug Design, Sch Pharm, Shanghai 200237, Peoples R China

年份：2026

卷号：14

期号：4

起止页码：1385

外文期刊名：JOURNAL OF MATERIALS CHEMISTRY B

收录：;EI(收录号：20260219897295);WOS:【SCI-EXPANDED(收录号:WOS:001659204200001)】；

基金：We thank the Public Instrument Service Platform of State Key Laboratory of Bioreactor Engineering and the Research Center of Analysis in this study. This study was supported by the National Key Research and Development Program of China (2018YFA0902804). And the National Natural Science Foundation of China (32471003).

语种：英文

外文关键词：Cartilage - Cell culture - Elastin - Hyaluronic acid - Hydrogels - Nanoparticles - Pathology - Repair - Transmission electron microscopy

摘要：Mesenchymal stem cell (MSC) transplantation has emerged as an effective approach for treating articular cartilage damage. However, unintended hypertrophy and fibrosis of the regenerated cartilage tissue markedly undermine the therapeutic effect. Herein, a two-factor delivery system, E60TK, based on the elastin-like protein ELP (VPGIG60, E60), co-delivering transforming growth factor beta 1 (TGF-beta 1) and kartogenin (KGN), was established to regulate stem cells from human exfoliated deciduous teeth (SHED)-directed cartilage differentiation and prevent cartilage hypertrophy and fibrosis. MTT and Alcian blue staining outcomes demonstrated that the fusion protein E60T composed of E60 and TGF-beta 1 was capable of effectively facilitating the proliferation and chondrogenic differentiation of SHED cells. Transmission electron microscope (TEM) and DLS findings revealed that the recombinant protein E60T could load KGN at physiological temperature to form nanoparticles E60TK, with particle sizes 241.2 +/- 46.1 nm. The results of immunofluorescence and immunohistochemistry following chondrogenic differentiation suggested that E60TK could efficiently induce the chondrogenic differentiation of SHED and significantly suppress the expression of type X collagen. The hyaluronic acid methacrylamide (HAMA) hydrogel loading E60TK, E60TK-HAMA, exhibited excellent biocompatibility, presented an interconnected porous architecture, and was completely degraded within 2 weeks in vitro. In vivo experiments indicated that the E60TK-HAMA hydrogel with SHED encapsulated could effectively promote the formation of new cartilage and inhibit hypertrophy. All these results signified that E60TK was an effective system for regulating the directed chondrogenic differentiation of SHED.