文献类型：期刊文献

中文题名：超滤法纯化超氧物岐化酶

英文题名：Purification of Superoxide Dismutase by Ultrafiltration

作者：赵铭[1];袁勤生[1];朱树新[1]

机构：[1]华东化工学院生化工程研究所

年份：1991

卷号：17

期号：4

起止页码：426

中文期刊名：华东化工学院学报

外文期刊名：Huadong Huagong Xueyuan xuebao

收录：国家哲学社会科学学术期刊数据库;EI(收录号：1992030424224);Scopus;CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：歧化酶;提纯;过滤膜

外文关键词：superoxide dismutase; ultrafiltration membrane; cellulose acetate; diafiltration

摘要：研制了适合纯化猪血超氧物岐化酶(Cu.Zn-SOD)溶液的醋酸纤维素(CA)超滤膜,其截留分子量约为16 000。研究了溶液浓度、操作压力等因素对通量的影响。结果表明,自制CA膜超滤纯化SOD溶液性能优于一些市售膜。收率为86～89%,比活提高25%,纯化因子1.35。利用透析过滤法,基本上能除去溶液中游离铜离子。
A cellulose acetate ultrafiltration membrane with molecular weight cut-off of 16000 was prepared and tested for the purification of pig blood superoxide dismutase (Cu. Zn-SOD) from its solution. The effects of the concentration and the operation pressure on flux were investigated. With a yield of 86～89%, a raise of specific activity of SOD by 25% and a purification rate of 1.35, the results showed that the CA ultrafiltration membrane was better than commerical ones. The free cupric ions in the solution were basically removed by diafiltration,