文献类型：期刊文献

英文题名：Preparation of optically pure tert-leucine by penicillin G acylase-catalyzed resolution

作者：Liu, SL; Song, QX; Wei, DZ; Zhang, YW; Wang, XD

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, New World Inst Biotechnol, Shanghai 200237, Peoples R China

年份：2006

卷号：36

期号：3

起止页码：235

外文期刊名：PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000237386800004)】；

语种：英文

外文关键词：hydrolysis; Kluyvera citrophila; penicillin G acylase; preparation; resolution; tert-leucine

摘要：Penicillin G acylase, from Kluyvera citrophila, was used in kinetic resolution of DL-tert-leucine. N-phenylacetylated-DL-tert-leucine, chemically synthesized from DL- tert -leucine, was enantioselctively hydrolyzed by penicillin G acylase to obtain L-tert-leucine, D-tert-leucine was prepared by acid-catalyzed hydrolysis of the remaining substrate. The total yields of D-tert-leucine and L-tert-leucine are 80.6% and 83.1%, respectively. The enantiomeric excess of the two products, D-tert-leucine and L-tert-leucine, are 98.5% and 99%. This is a practical way for the preparation of D-tert-leucine and L-tert-leucine.