文献类型：期刊文献

中文题名：小麦胚芽肽和獐牙菜苦苷对紫外线B诱导的小鼠日光性皮炎的协同保护作用

英文题名：Synergistic Protective Effects of Wheat Germ Peptides and Swertimarin Against Ultraviolet B-induced Solar Dermatitis in Mice

作者：马悦文[1,2,3];陶沁倩[4];朱超凡[4];严二平[4];陈贤[5];王春丽[1,2,3]

机构：[1]华东理工大学药学院,上海200237;[2]制药工程与过程化学教育部工程研究中心,上海200237;[3]上海市新药设计重点实验室,上海200237;[4]广州天然国度生物科技有限公司,广州511300;[5]广州伽能生物科技有限公司,广州511335

年份：2025

卷号：42

期号：18

起止页码：3140

中文期刊名：中国现代应用药学

外文期刊名：Chinese Journal of Modern Applied Pharmacy

收录：;北大核心:【北大核心2023】；

基金：国家科技重大专项(2018ZX09735002)。

语种：中文

中文关键词：小麦胚芽肽;獐牙菜苦苷;紫外线B;日光性皮炎;抗氧化;抗炎;协同作用

外文关键词：wheat germ peptide;swertiamarin;ultraviolet B;solar dermatitis;antioxidant;anti-inflammatory;cooperative interaction

摘要：目的 研究小麦胚芽肽和獐牙菜苦苷对紫外线B(ultraviolet B,UVB)诱导的日光性皮炎小鼠的协同保护作用。方法 将KM小鼠随机分为6组(n=12):空白组、模型组、康复新液组(KFXY)、 40 mg·kg^(-1)小麦胚芽肽组(DSM1)、40 mg·kg^(-1)獐牙菜苦苷组(DSM2)和20 mg·kg^(-1)小麦胚芽肽+20 mg·kg^(-1)獐牙菜苦苷组(DSM3)。空白组小鼠仅进行剃毛,不照射,其余组进行UVB照射。给药组均外用给药,共干预7 d。实验结束后,拍照记录小鼠的皮肤状态,对皮肤组织进行苏木精-伊红(HE)染色、苦味酸-酸性品红法(VG)染色。通过生化试验检测皮肤组织中氧化应激指标T-AOC、NOS、NO、MDA的含量及活力水平,通过免疫组化检测皮肤组织中炎症指标TNF-α、IL-6、IL-1β水平,通过qRT-PCR检测皮肤组织中NQO1、GCLC、Nrf2的mRNA水平,通过Western blotting检测皮肤组织中NQO1、GCLC、Nrf2的蛋白水平。结果 与模型组对比,DSM1、DSM2、DSM3组小鼠皮肤损伤程度有所改善,胶原纤维排列整齐,胶原密度升高,TAOC水平提高,NOS、NO、MDA水平降低,TNF-α、IL-6、IL-1β表达水平降低,皮肤组织中NQO1、GCLC、Nrf2的m RNA表达水平和蛋白相对表达量升高。结论 小麦胚芽肽与獐牙菜苦苷联用可改善UVB介导的小鼠日光性皮炎,推测其作用机制与激活Nrf2信号通路有关。
OBJECTIVE To study the synergistic protective effects of wheat germ(Triticum aestivum)peptides and swertimarin in mice with ultraviolet B(UVB)-induced solar dermatitis.METHODS KM mice were randomly divided into 6 groups(n=12):blank group,model group,rehabilitated new group(KFXY),40 mg·kg^(?1) wheat germ peptide group(DSM1),40 mg·kg^(?1) swertiamarin group(DSM2),and 20 mg·kg^(?1) wheat germ peptide+20 mg·kg^(?1) swertiamarin group(DSM3).Mice in the blank group were only shaved and not irradiated,while the remaining groups were irradiated with UVB.The drug delivery groups received topical application for 7 d.At the end of the experiment,photos were taken to record the skin status of the mice,and the skin tissues were stained with hematoxylin-eosin staining(HE),picric acid-acidic magenta method(VG).The content and viability levels of oxidative stress indicators T-AOC,NOS,NO and MDA in skin tissues were detected by biochemistry,the levels of inflammatory indicators TNF-α,IL-6 and IL-1βin skin tissues were detected by immunohistochemistry,the mRNA levels of NQO1,GCLC and Nrf2 in skin tissues were detected by qRT-PCR.The protein levels of NQO1,GCLC and Nrf2 in skin tissues were detected by Western blotting.RESULTS Compared with the model group,the degree of skin damage in the DSM1,DSM2 and DSM3 groups was improved,the collagen fibers were neatly arranged,the collagen density were increased,the levels of T-AOC were increased,the levels of NOS,NO and MDA were decreased,the expression levels of TNF-α,IL-6 and IL-1βwere decreased,the mRNA and protein expression levels of NQO1,GCLC and Nrf2 were increased.CONCLUSION Wheat germ peptide and swertiamarin in combination ameliorated UVB-mediated solar dermatitis in mice,which may be related to activation of the Nrf2 pathways.