文献类型：期刊文献

英文题名：Using algae cells to drive cofactor regeneration and asymmetric reduction for the synthesis of chiral chemicals

作者：Zheng, Lvhong[1,2];Zhang, Xiaoyan[1,3];Bai, Yunpeng[1,3];Fan, Jianhua[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Dept Appl Biol, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Dept Bioengn, Shanghai 200237, Peoples R China

年份：2018

卷号：35

起止页码：432

外文期刊名：ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000447187700047)】；

基金：This work was sponsored by National Key Research and Development Program of China (2016YFA0204300), Natural Science Foundation of Shanghai 18ZR1410100, 18ZR1409900 and 17ZR1406700, Shanghai Pujiang Program (18PJD008), National Natural Science Foundation of China 21505044 and 21506055, and Open Funding Project of the State Key Laboratory of Bioreactor Engineering.

语种：英文

外文关键词：Algae biocatalyst; Chiral alcohols; Biocatalysis; Cell factory; Microalgae; Artificial modification

摘要：Asymmetric reduction of prochiral ketones via biocatalysis is the most effective method for the synthesis of chiral alcohols. The industrial biocatalytic reduction process requires additional co-substrates and enzymes, e.g., glucose and glucose dehydrogenase, to regenerate reductive cofactors, such as nicotinamide adenine dinucleotide (NADH) and nicotinamide adenine dinucleotide phosphate (NADPH), for efficient substrate conversion and product yield. Thus, increasing the cost in reaction and downstream treatment. Recently, algae cells, due to a combination of microorganism-like properties (rapid growth and high density culture) and plant-like properties (highly active photosynthesis), have attracted much attention as novel host cells in industrial biocatalysis. Particularly, autotrophic photosynthesis in algae is capable of regenerating NADH and NADPH rapidly, eliminating the requirement of additional enzymes and co-substrates for cofactor regeneration in normal biocatalytic processes. Recently, a variety of algae species, such as cyanobacteria, green algae, diatoms, chrysophytes, and red algae, have been applied in asymmetric biocatalysis. Herein, various asymmetric reduction reactions performed by different type of algae cells have been reviewed, and methods for improving the reaction efficiency have been discussed. We aim to attract algae microbiologists and chemists to push forward the study of algae as biocatalytic cell factories for value-added industrial chemical production in the future.