文献类型：期刊文献

英文题名：Antibacterial Activity and Mechanism of the Sesquiterpene Δ-Cadinene Against Listeria Monocytogenes and its Application in Milk

作者：Qin, Rong[1]; Yang, Shengping[1]; Fu, Bin[1]; Chen, Yang[1]; Qi, Yonggang[1]; Xu, Ning[1]; Wu, Qian[1]; Hua, Qiang[2]; Wu, Yongkang[3]; Liu, Zhijie[1]

机构：[1] Key Laboratory of Fermentation Engineering [Ministry of Education], Cooperative Innovation Center of Industrial Fermentation [Ministry of Education & Hubei Province], National "111" Center for Cellular Regulation and Molecular Pharmaceutics, Hubei Key Laboratory of Industrial Microbiology, Hubei University of Technology, Wuhan, 430068, China; [2] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China; [3] Hubei Juhui Agriculture Co., Ltd, Jingmen, 431821, China

年份：2023

外文期刊名：SSRN

收录：EI(收录号：20230335709)

语种：英文

外文关键词：Biofilms - Cytology - Diseases - Gene expression - Gene Ontology - Listeria - Nucleic acids

摘要：Listeria monocytogenes is a food-borne pathogen that seriously threatens human health. We analyzed the antibacterial activity and mechanism of δ-cadinene against L. monocytogenes. The effect of δ-cadinene on L. monocytogenes gene expression was investigated using RNA-sequencing. δ-Cadinene inhibited L. monocytogenes growth with a minimum inhibitory concentration (MIC) of 5 μL/mL and a minimum bactericidal concentration of 10 μL/mL. δ-Cadinene suppressed L. monocytogenes biofilm formation, altered cell morphology, and damaged the cell membrane, enhancing membrane permeability and affecting the membrane potential. "Plasma membrane" and "membrane" were the most significantly enriched Gene Ontology terms. Sixteen differentially expressed genes were strongly correlated with biofilm formation, protein and nucleic acid leakage, and membrane potential. L. monocytogenes growth in milk at 4 °C was well controlled with 5 μL/mL δ-cadinene. In conclusion, treatment with δ-cadinene could be a potential strategy to control L. monocytogenes growth in foods. ? 2023, The Authors. All rights reserved.