文献类型：期刊文献

英文题名：Engineering synthetic artificial pancreas using chitosan hydrogels integrated with glucose-responsive microspheres for insulin delivery

作者：Yin, Ruixue[1,2];He, Jing[1];Bai, Meirong[3,4];Huang, Cong[1];Wang, Kemin[2,5];Zhang, Hongbo[1];Yang, Shih-Mo[6];Zhang, Wenjun[2,6]

机构：[1]East China Univ Sci & Technol, Complex & Intelligent Syst Res Ctr, Shanghai, Peoples R China;[2]Univ Saskatchewan, Div Biomed Engn, Saskatoon, SK, Canada;[3]Beijing Univ Chem Technol, State Key Lab Chem Resource Engn, Beijing, Peoples R China;[4]Beijing Univ Chem Technol, Beijing Lab Biomed Mat, Beijing, Peoples R China;[5]Changzhou Univ, Sch Mat Sci & Engn, Changzhou, Peoples R China;[6]Shanghai Univ, Sch Mechatron & Automat, Shanghai, Peoples R China

年份：2019

卷号：96

起止页码：374

外文期刊名：MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS

收录：;EI(收录号：20184906206260);WOS:【SCI-EXPANDED(收录号:WOS:000456760700039)】；

基金：The authors would like to thank the National Natural Science Foundation of China (21404038) and the Fundamental Research Funds for the Central Universities (22A201514029) for the financial support to this work.

语种：英文

外文关键词：Artificial pancreas; Glucose-responsive; Insulin delivery; Prolonged release; Integrated scaffold

摘要：The closed-loop delivery of insulin in response to change of the blood glucose level and long-term supply of insulin are both important for diabetes patients. However, combination of these two goals in a chemically controlled implantable system is still challenging yet highly desirable. The purpose of the present study is to design a synthetic artificial pancreas by integration of chitosan hydrogels with insulin-loaded glucose-responsive microspheres to deliver insulin in a close-looped and long-term way for diabetes care. Glucose-responsive insulin-loaded microspheres were firstly fabricated via a high-speed shear-emulsion based crosslinking method and then embedded into chitosan hydrogels to make a scaffold-based synthetic artificial pancreas. In vitro experiments indicated the scaffold exhibited a longer insulin supply as well as a lower burst release compared with free microspheres, and could keep the glucose-responsive insulin release property inherited from the corresponding microspheres even after 12 day-release. The released insulin was proved to remain active, and the culture of HDF cells on the scaffold showed good cell proliferation during 7 days incubation. These results suggested the scaffold-based synthetic artificial pancreas have great promise in the application of insulin delivery.