文献类型：期刊文献

中文题名：多巴胺端基聚磺基甜菜碱的表面改性及其表面抗蛋白吸附性能

英文题名：Dopamine-Based Poly(sulfubetaine methacrylate)Surface Modification and Its Antifouling Properties

作者：龚伟[1];牛亚鹏[1];韩霞[1];刘洪来[1]

机构：[1]华东理工大学化学与分子工程学院,上海200237

年份：2018

卷号：44

期号：3

起止页码：316

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD_E2017_2018】；

基金：国家自然科学基金(91534103)

语种：中文

中文关键词：聚磺基甜菜碱;多巴胺;表面改性;抗蛋白吸附

外文关键词：poly（sulfubetaine methacrylate）;dopamine;surface modification;antifouling

摘要：将具有黏附性的多巴胺基团和具有亲水抗污性的聚甲基丙烯酸磺基甜菜碱(PSBMA)结合,利用原子转移自由基聚合方法合成了多巴胺(DOPA)端基修饰的两性离子聚合物DOPA-PSBMA,并且通过简便的聚合物溶液浸涂方式对硅片进行表面改性。研究发现,DOPA-PSBMA具有温度响应性,其高临界溶解温度(UCST)为22~35℃;该聚合物可通过溶液自组装有效黏附于硅片表面,且在37℃和4℃时,改性硅片均具有良好的抗蛋白吸附性能,说明温度对DOPA-PSBMA改性表面的抗蛋白吸附性能基本无影响。
Undesired biofouling is always considered as one of the most important factors that affects the efficiency,durability,or even the workability of materials applied in biomedical and industrial field,including biosensors,implant devices,drug delivery carriers,filtration membranes,marine vessels,and so on.To address this issue,it is quite necessary to develop fouling-resistant materials by introducing antifouling polymers on surface.Zwitterionic polymers,based on carboxybetaine,sulfobetaine or phosphorylcholine,are widely applied in surface modification to obtain nonfouling materials due to their unique biological antifouling properties.The traditional surface modification strategies,including ＂graft to＂and＂graft from＂,both involve sophisticated functionalization of surface.Only pretreated surfaces with anchoring groups or initiators that allow the antifouling polymers for durable modification.Therefore,the design of a simple and effective surface modification method is of great significance to the materials resisting nonspecific protein adsorption.Inspired by the strong adhesion of mussel protein,dopamine,the crucial component for adhesion,is chosen as an anchoring group that can tightly bind to the substrate without surface chemical specificity.In this work,the catechol（DOPA）group with great adhesion and poly（sulfubetaine methacrylate）（PSBMA）with excellent protein resistance properties were conjugated together to obtain an adhesive zwitterionic polymer DOPA-PSBMA to be used as the antifouling surface modifier.DOPA-PSBMA was successfully synthesized by an atomic transfer radical polymerization（ATRP）method.DOPA-PSBMA is a temperature-responsive polymer with an upper critical solution temperature of 22—35℃.DOPA-PSBMA was successfully attached on the surface of hydroxylated silicon substrate from polymer solution dipping at room temperature,which can be proved by the X-ray photoelectron spectroscopy（XPS）.Thus,the antifouling properties of the silicon substrates were greatly improved after attachment of DOPA-PSBMA.From both laser scanning confocal microscopy（LSCM）and ELISA technique,the antifouling properties of the pristine and DOPA-PSBMA modified substrates were compared.The modified silicon substrates had good resistance to protein adsorption performance under 37℃ and 4℃,indicating that the antifouling properties of DOPA-PSBMA modified surface was temperatureinsensitive.The facile method to modify substrate surfaces to be antifouling by the DOPA-PSBMA solution dipping will be of great potential application in surface engineering and biomaterial science.