文献类型：期刊文献

英文题名：Development and Application of an Efficient Medium for Chromogenic Catalysis of Tetramethylbenzidine with Horseradish Peroxidase

作者：Li, Meng[1];Su, Haying[1];Tu, Yan[1];Shang, Yazhuo[1];Liu, Yu[2,3];Peng, Changjun[1];Liu, Honglai[1]

机构：[1]East China Univ Sci & Technol, Key Lab Adv Mat, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Chem Engn, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Sch Chem Engn, Shanghai 200237, Peoples R China

年份：2019

卷号：4

期号：3

起止页码：5459

外文期刊名：ACS OMEGA

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000462921900101)】；

基金：This work is supported by the National Natural Science Foundation of China (Project Nos. 21476072 and 21776070), the Fundamental Research Funds for the Central Universities and the National Basic Research Program of China (973 Program), 2015CB251401.

语种：英文

摘要：The alkylimidazolium tetrafluoroborate ionic liquids (ILs) ([C(n)mim][BF4] n = 2, 4, 6, 8, 10) and anionic surfactant sodium dodecyl sulfate (SDS) were combined together to produce effective mediums for chromogenic catalysis of tetramethylbenzidine (TMB) with horseradish peroxidase (HRP) in the presence of H2O2. The chromogenic performance, kinetic behavior, and the possible influencing mechanism for the chromogenic catalysis of HRP-H2O2-TMB were discussed in detail. Therein, the roles of ionic liquids (ILs) were highlighted by the combination of experiments and theoretical calculations. The SDS/[C(4)mim][BF4]combination displayed superiority in chromogenic catalysis by improving both the substrate solubility and product stability to the maximum extent possible. Furthermore, SDS/[C(4)mim][BF4] combination showed uniqueness for TMB in improving the chromogenic performance compared with other chromogenic substrates of HRP. Inspired by the efficient chromogenic system, an enhanced enzyme-linked immunosorbent assay strategy for the detection of human immunoglobulin G was established and the sensitive colorimetric strategies for the detection of H2O2 and glucose were further developed by employing SDS/[C(4)mim][BF4] combination as the medium of chromogenic catalysis of HRP-H2O2-TMB. This unique chromogenic system is endowed with multitude of potential applications in biological systems.