文献类型：期刊文献

英文题名：Segmental bone regeneration using rhBMP-2-loaded collagen/chitosan microspheres composite scaffold in a rabbit model

作者：Hou, Juan[1];Wang, Jing[1];Cao, Lingyan[1];Qian, Xiuzhen[1];Xing, Wanli[1];Lu, Jingxiong[1];Liu, Changsheng[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Key Lab Ultrafine Mat, Engn Res Ctr Biomed Mat,Minist Educ, Shanghai 200237, Peoples R China

年份：2012

卷号：7

期号：3

外文期刊名：BIOMEDICAL MATERIALS

收录：;EI(收录号：20121114858789);WOS:【SCI-EXPANDED(收录号:WOS:000303667600002)】；

基金：The authors wish to express their gratitude for financial support from National Basic Research Program of China (973 Program, 2012CB933600), the State Key Program of National Natural Science of China (no 50732002), National Natural Science Foundation of China (nos 50973029 and 50873034), Program for Chang Jiang Scholars and Innovative Research Team (IRT 0825) and the scientific research foundation for the excellent young scholars from ECUST (no YD0157109). They also acknowledge the orthopaedic department of Xijing hospital-Fourth Military Medical University for the micro-CT technical assistance.

语种：英文

外文关键词：Bone - Recombinant proteins - Collagen - Scaffolds (biology) - Microspheres

摘要：The reconstruction of segmental bone defects remains an urgent problem in the orthopaedic field, and bone morphogenetic protein-2 (BMP-2) is known for its potent osteoinductive properties in bone regeneration. In this study, chitosan microspheres (CMs) were prepared and combined with absorbable collagen sponge to maintain controlled-release recombinant human bone morphogenetic protein-2 (rhBMP-2). The rhBMP-2-loaded composite scaffolds were implanted into 15 mm radius defects of rabbits and the bone-repair ability was evaluated systematically. CMs were spherical in shape and had a polyporous surface, according to SEM images. The complex scaffold exhibited an ideal releasing profile in vitro. The micro-computed tomographic analysis revealed that the rhBMP-2-loaded composite scaffold not only bridged the defects as early as 4 weeks, but also healed the defects and presented recanalization of the bone-marrow cavity at 12 weeks. These results were confirmed by x-ray. When compared with other control groups, the composite scaffold group remarkably enhanced new bone formation and mechanical properties, as evidenced by bone mineral content evaluation, histological observations and biomechanical testing. Moreover, the biocompatibility and appropriate degradation of the composite scaffold could be obtained. All of these results clearly demonstrated that the composite scaffold is a promising carrier of BMP-2 for the treatment of segmental bone defects.