文献类型：期刊文献

英文题名：Efficient production and characterization of homopolymeric poly(3-hydroxyvalerate) produced by Bacillus strain PJC48

作者：Gu, Jin-Jin[1];Zhou, Ying[2];Tong, Yan-Bin[1];Lu, Jian-Jiang[1];Ye, Bang-Ce[1,2]

机构：[1]Shihezi Univ, Sch Chem & Chem Engn, Key Lab Green Proc Chem Engn Xinjiang Bingtuan, Shihezi 832000, Xinjiang, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2018

卷号：65

期号：4

起止页码：622

外文期刊名：BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY

收录：;EI(收录号：20180804822946);WOS:【SCI-EXPANDED(收录号:WOS:000441743500014)】；

基金：This research was supported by the National Natural Science Foundation of China (21575089) and Fundamental Research Funds for the Central Universities (222201714051). The authors are very grateful for the financial assistance. The authors declare no conflicts of interest.

语种：英文

外文关键词：Bacillus species; biopolymers; fermentation; nuclear magnetic resonance; response surface methodology

摘要：Aliphatic polyester, poly(3-hydroxyvalerate) (PHV), is commonly produced as a granular component in bacterial cells of various species. Based on 16S rDNA gene sequence analysis, strain PJC48 was identified as a Bacillus species. The current study is aimed to screen for a high-yield strain that can produce PHV efficiently and to increase PHV product yield by optimizing the fermentative process. We identified a high-producer strain based on Nile red staining. Characterization of the PHV produced by PJC48 by nuclear magnetic resonance spectroscopy revealed that it consisted of (R)-3-hydroxyvalerate monomers. The suggested model was validated by response surface methodology. Optimization of the PHV yield resulted in an increase of 32.75% compared to control, with a maximum production of 1.64 g/L after 48 H.