文献类型：期刊文献

英文题名：Identification of a CqCaspase gene with antiviral activity from red claw crayfish Cherax quadricarinatus

作者：li, Yan-yao[1,2,3];Xie, Xiao-lu[3];Ma, Xing-yuan[1,2];Liu, Hai-peng[3]

机构：[1]East China Univ Sci & Technol, Sch Biotechnol, Shanghai, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[3]Xiamen Univ, Fujian Engn Lab Marine Bioprod & Technol, Fujian Collaborat Innovat Ctr Exploitat & Utiliza, State Key Lab Marine Environm Sci, Xiamen 361102, Fujian, Peoples R China

年份：2019

卷号：91

起止页码：101

外文期刊名：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000451492900011)】；

基金：This work was supported by the National Natural Science Foundation of China (nos. U1605214, 41476117, 41676135) and FRFCU (20720180123, 20720162010, 20720170083, 2017X0622).

语种：英文

外文关键词：CqCaspase; Antiviral activity; Cherax quadricarinatus; White spot syndrome virus

摘要：Caspase, an aspartate specific proteinase mediating apoptosis, plays a key role in immune response. In our previous study, the expression of a caspase gene was up-regulated in a transcriptome library from the haematopoietic tissue (Hpt) cells of red claw crayfish Cherax quadricarinatus post white spot syndrome virus (WSSV) infection. To further reveal the effect of caspase on WSSV infection, we cloned this caspase gene (denominated as CqCaspase) with an open reading frame of 1062 bp, which encoded 353 amino acids with a caspase domain (CASc) containing a p20 subunit and a p10 subunit. Tissue distribution analysis indicated that the tnRNA transcript of CqCaspase was widely expressed in all tested tissues with the highest expression in Hpt, while the lowest expression in muscle. To further explore the effect of CqCaspase on WSSV replication, recombinant protein of CqCaspase (rCqCaspase) was delivered into Hpt cells followed by WSSV infection, which resulted in a significantly decreased expression of both an immediate early gene IE1 and a late envelope protein gene VP28 of WSSV, suggesting that CqCaspase, possibly by the enhanced apoptotic activity, had a strong negative effect on the WSSV replication. These data together indicated that CqCaspase was likely to play a vital role in immune defense against WSSV infection in a crustacean C. quadricarinatus, which shed a new light on the mechanism study of WSSV infection in crustaceans.