文献类型：期刊文献

英文题名：A cytokine receptor domeless promotes white spot syndrome virus infection via JAK/STAT signaling pathway in red claw crayfish Cherax quadricarinatus

作者：Liu, Ling-ke[1];Chen, Xiao-xiao[1,2];Gao, Rui-lin[1];Wang, Ke-jian[1];Zheng, Wen-yun[2];Liu, Hai-peng[1]

机构：[1]Xiamen Univ, Coll Ocean & Earth Sci, State Key Lab Marine Environm Sci, State Prov Joint Engn Lab Marine Bioprod & Techno, Xiamen 361102, Fujian, Peoples R China;[2]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China

年份：2020

卷号：111

外文期刊名：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000551873700016)】；

基金：This study was supported by the Natural Science Foundation of China (Nos. U1605214, 41676135), Fundamental Research Funds for the Central Universities of China (No. 20720180123) and Entrepreneurship Training Programs (20720162010, 20720170083, 2017X0622) and science and technology innovation action plan of Shanghai, China (17431904600).

语种：英文

外文关键词：Domeless; White spot syndrome virus; JAK/STAT pathway; Cherax quadricarinatus; Phosphorylation

摘要：The Janus kinase/signal transducers and activators of transcription (JAK/STAT) signaling pathway is pivotal in immune responses for a variety of pathogens in both vertebrates and invertebrates. Domeless (Dome), as a unique cytokine receptor, involves in the upstream JAK/STAT pathway in invertebrates. In this study, the full-length cDNA sequence of a cytokine receptor Dome was identified from red claw crayfish Cherax quadricarinatus (named as CaDome), which contained an open reading frame of 4251 bp, encoding 1416 amino acids. The CqDome contained extracellular conservative domains of a signal peptide, two cytokine binding modules (CBM), three fibronectin-type-III-like (FN3) domains and a transmembrane region. Tissue distribution analysis showed that CqDome generally expressed in all the tissues selected with a high expression in hemocyte. The gene expression of both the viral immediately early gene (IE1) and a late gene envelope protein VP28 of white spot syndrome virus (WSSV) were significantly decreased after gene silencing of CqDome in crayfish haematopoietic tissue (Hpt) cells, indicating a key role of CqDome in promoting WSSV infection. Furthermore, the phosphorylation level of CqSTAT was significantly inhibited by gene silencing of CqDome in Hpt cells, indicating that CqDome participated in signal transduction of JAK/STAT pathway in red claw crayfish. These data together suggest that CqDome is likely to promote WSSV infection via JAK/STAT pathway, which sheds new light on further elucidation of the pathogenesis of WSSV.