文献类型：期刊文献

英文题名：Iron Phthalocyanine Decorated Nitrogen-Doped Graphene Biosensing Platform for Real-Time Detection of Nitric Oxide Released from Living Cells

作者：Xu, Huiying[1];Liao, Chong[1];Liu, Yujie[2,3];Ye, Bang-Ce[1];Liu, Baohong[2,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Biosyst & Microanal, Shanghai 200237, Peoples R China;[2]Fudan Univ, Collaborat Innovat Ctr Chem Energy Mat, Dept Chem, Shanghai 200433, Peoples R China;[3]Fudan Univ, State Key Lab Mol Engn Polymers, Shanghai 200433, Peoples R China

年份：2018

卷号：90

期号：7

起止页码：4438

外文期刊名：ANALYTICAL CHEMISTRY

收录：;EI(收录号：20181504995795);WOS:【SCI-EXPANDED(收录号:WOS:000429385800030)】；

基金：This work was supported by the National Natural Science Foundation of China (Grant Nos. 21705047 and 21335003), the Chinese Post-Doctoral Fund (Grant No. 2017M621380), and the Fundamental Research Funds for the Central Universities (Grant No. 222201814030).

语种：英文

外文关键词：Biological systems - Endothelial cells - Nanocomposites - Doping (additives) - Amino acids - Iron compounds - Signal detection - Biocompatibility - Real time systems - Graphene - Iron - Nitrogen

摘要：Nitric oxide (NO) is a transcellular messenger involved in many physiological and pathological processes, but the real-time detection of NO in biological systems is still challenging due to its rapid diffusion, low concentration, and short half-life. A novel electrochemical sensing platform based on iron phthalocyanine (FePc) functionalized nitrogen-doped graphene (N-G) nanocomposites was constructed to achieve in situ monitoring of NO released from living cells on the sensing layer. By taking advantage of the synergetic effect of N-G and FePc nanocomposites, the N-G/FePc sensor displays excellent electrocatalytic activity toward NO with a high sensitivity of 0.21 mu A mu M-1 cm(-2) and a low detection limit of 180 nmol L-1. The following layer-by-layer assembly of poly-L-lysine (PLL) and Nafion further improved the capacity of resisting disturbance as well as the biocompatibility of the sensing interface. The flexible design of the ITO substrate based electrode provides a more controlled cellular biosensing system which could capture molecular signals immediately after NO released from human umbilical vein endothelial cells (HUVECs). The exhibited additional features of high sensitivity, rapid response, and ease of operation implies that the proposed N-G/FePc/Nafion/PLL ITO biosensor is a promising powerful platform in various complex biological systems.