文献类型：期刊文献

英文题名：Poly(I:C) induces anti-inflammatory response against secondary LPS challenge in zebrafish larvae

作者：Wang, Zhuang[1];You, Xinwei[1];Zhang, Yuanxing[2,3];Liu, Qin[1,3];Yang, Dahai[1,3,4]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Aquat Anim Dis, Shanghai 200237, Peoples R China;[2]Southern Marine Sci & Engn Guangdong Lab Zhuhai, Zhuhai 519000, Peoples R China;[3]Shanghai Engn Res Ctr Maricultured Anim Vaccines, Shanghai 200237, Peoples R China;[4]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2024

卷号：144

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001138075200001)】；

基金：This work was supported by the National Natural Science Foundation of China (32122090 to D.Y., 32102836 to Z.W.) , and Fundamental Research Funds for the Central Universities (222201231733 to Y.Z.) .

语种：英文

外文关键词：Poly(I:C); Macrophages; Anti -inflammation; Septic shock; Zebrafish larvae

摘要：Poly(I:C) is known as an agonist of the TLR3 receptor which could prime inflammation and elicit the host immune response, which is widely applied as adjuvant or antivirus treatment. However, the negative effects of poly(I:C) on regulating immune response to protect the host from inflammatory diseases remain largely unknown. Here, we establish an in vivo model to pre-treat zebrafish larvae with poly(I:C) at 2 dpf, then challenge them with LPS at 6 dpf, and find that poly(I:C) training could significantly alleviate the LPS challenge-induced septic shock and inflammatory phenotypes. Moreover, the poly(I:C)-trained larvae exhibit decreased number of macrophages, but not neutrophils, after secondary LPS challenge. Furthermore, training the larvae with poly(I:C) could elevate the transcripts of mTOR signaling and heighten the H3K4me3-mediated epigenetic modifications. And interestingly, we find that inhibiting the H3K4me3 modification, rather than mTOR signaling, could recover the number of macrophages in poly(I:C)-trained larvae, which is consistent with the observations of inflammatory phenotypes. Taken together, these results suggest that poly(I:C) training could induce epigenetic rewiring to mediate the anti-inflammatory response against secondary LPS challenge-induced septic shock through decreasing macrophages' number in vivo, which might expand our understanding of poly(I:C) in regulating fish immune response.