文献类型：期刊文献

英文题名：Recent advances in solid-state nuclear magnetic resonance studies on membrane fusion proteins

作者：Zheng, Lifen[1];Wang, Shenlin[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2025

卷号：292

期号：3

起止页码：483

外文期刊名：FEBS JOURNAL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001356868000001)】；

基金：This work was supported by the National Natural Science Foundation of China (grant number: 22274050), the Shanghai Science and Technology Commission (contract number: 23J21900300) and the Fundamental Research Funds for the Central Universities.

语种：英文

外文关键词：enveloped virus; membrane fusion; membrane fusion protein; saposin-like proteins family; solid-state NMR

摘要：Membrane fusion is an essential biological process that merges two separate lipid bilayers into a whole one. Membrane fusion proteins facilitate this process by bringing lipid bilayers in close proximity to reduce the repulsive energy between membranes. Along with their interactions with membranes, the structures and dynamics of membrane fusion proteins are key to elucidating the mechanisms of membrane fusion. Solid-state NMR (SSNMR) spectroscopy has unique advantages in determining the structures and dynamics of membrane fusion proteins in their membrane-bound states. It has been extensively applied to reveal conformational changes in intermediate states of viral membrane fusion proteins and to characterize the critical lipid-membrane interactions that drive the fusion process. In this review, we summarize recent advancements in SSNMR techniques for studying membrane fusion proteins and their applications in elucidating the mechanisms of membrane fusion.