文献类型：期刊文献

英文题名：Spatiotemporal monitoring of NAD+ metabolism with fluorescent biosensors

作者：Chen, Weicai[1,3];Liu, Shuning[1,3];Yang, Yi[1];Zhang, Zhuo[1,2,3];Zhao, Yuzheng[1,2,3]

机构：[1]East China Univ Sci & Technol, Optogenet & Synthet Biol Interdisciplinary Res Ct, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Me, State Key Lab Bioreactor Engn, 130 Mei Long Rd, Shanghai 200237, Peoples R China;[2]Chinese Acad Med Sci, Res Unit New Tech Live Cell Metab Imaging, Beijing 100730, Peoples R China;[3]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, 130 Mei Long Rd, Shanghai 200237, Peoples R China

年份：2022

卷号：204

外文期刊名：MECHANISMS OF AGEING AND DEVELOPMENT

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000792904400006)】；

基金：& nbsp;This research is supported by National Key Research and Develop-ment Program of China (2019YFA0904800 to Y.Zhao) , NSFC (32150030, 32030065, 32121005, 92049304 to Y.Zhao) , Research Unit of New Techniques for Live-cell Metabolic Imaging (Chinese Academy of Medical Sciences, 2019RU01, 2019-I2M-5-013 to Y.Zhao) , Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism (Y.Z.) , Innovative research team of high-level local universities in Shanghai, the Fundamental Research Funds for the Central Universities.

语种：英文

外文关键词：NAD plus; Genetically encoded fluorescent sensors; Semisynthetic fluorescent sensors; Real-time monitoring; Fluorescence imaging

摘要：Nicotinamide adenine dinucleotide (NAD+) plays vital roles in cell metabolism, cell signaling, and gene expression regulation. NAD+ shows intricate subcellular distribution and dynamic changes in diverse biological processes; however, traditional biochemical assays usually require cell lysis, making it technically difficult to measure NAD(+) metabolism in live cells or in vivo. Recently, a few genetically encoded and semisynthetic fluorescent sensors have been used to monitor NAD(+) metabolism in a variety of biological settings. In this review, we summarize the recent progress in the development of fluorescent sensors for NAD(+) and their applications in life science research. We further analyze their advantages, limitations, and perspectives for future development.