文献类型：期刊文献

英文题名：The impact of antifouling layers in fabricating bioactive surfaces

作者：Chen, Qi[1];Zhang, Donghui[2];Gu, Jiawei[2];Zhang, Haodong[2];Wu, Xue[2];Cao, Chuntao[2];Zhang, Xinyue[2];Liu, Runhui[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Frontiers Sci Ctr Materiobiol & Dynam Chem, Res Ctr Biomed Mat, Sch Mat Sci & Engn,Key Lab Ultrafine Mat,Minist E, Shanghai 200237, Peoples R China

年份：2021

卷号：126

起止页码：45

外文期刊名：ACTA BIOMATERIALIA

收录：;EI(收录号：20215111338634);WOS:【SCI-EXPANDED(收录号:WOS:000646156300004)】；

基金：This research was supported by the National Natural Science Foundation of China (no. 21774031 , 31800801) , the Natural Science Foundation of Shanghai (18ZR1410300) , the National Natural Science Foundation of China for Innovative Research Groups (no. 51621002) , the National Key Research and Development Program of China (2016YFC1100401) , Program of Shanghai Academic/Technology Research Leader (20XD1421400) , Research Program of State Key Laboratory of Bioreactor Engineering, and the Fundamental Research Funds for the Central Universities (22221818014) .

语种：英文

外文关键词：Fouling; Antifouling; PEG; Bioactive surface; Cell adhesion; Functional peptides

摘要：The nonspecific protein adsorption and cell adhesion will cause unwanted background ?noise? on the surface of biological materials and detecting devices and compromise the performance of functional molecules and, therefore, impair the performance of materials and the sensitivity of devices. In addition, the selection of antifouling surfaces with proper chain length and high grafting density is also of great importance and requires further studies. Otherwise, the surface-tethered bioactive molecules may not function in their optimal status or even fail to display their functions. Based on these two critical issues, we summarize antifouling molecules with different structures, variable grafting methods, and diverse applications in biomaterials and biomedical devices reported in literature. Overall, we expect to shed some light on choosing the appropriate antifouling molecules in fabricating bioactive surfaces. Bioactive surfaces modified with functional peptides are critical for both fundamental research and prac-tical application of implant materials and tissue repair. However, when bioactive molecules are tethered on biomaterial surfaces, their functions can be compromised due to unwanted fouling (mainly nonspecific protein adsorption and cell adhesion). In recent years, researchers have continuously studied antifouling strategies to obtain low background noise and effectively present the function of bioactive molecules. In this review, we describe several commonly used antifouling strategies and analyzed their advantages and drawbacks. Among these strategies, antifouling molecules are widely used to construct the antifoul -ing layer of various bioactive surfaces. Subsequently, we summarize various structures of antifouling molecules and their surface grafting methods and characteristics. Application of these functionalized sur-faces in microarray, biosensors, and implants are also introduced. Finally, we discuss the primary chal-lenges associated with antifouling layers in fabricating bioactive surfaces and provide prospects for the future development of this field. Statement of significance The nonspecific protein adsorption and cell adhesion will cause unwanted background ?noise? on the surface of biological materials and detecting devices and compromise the performance of functional molecules and, therefore, impair the performance of materials and the sensitivity of devices. In addi-tion, the selection of antifouling surfaces with proper chain length and high grafting density is also of great importance and requires further studies. Otherwise, the surface-tethered bioactive molecules may not function in their optimal status or even fail to display their functions. Based on these two critical issues, we summarize antifouling molecules with different structures, variable grafting methods, and di-verse applications in biomaterials and biomedical devices reported in literature. Overall, we expect to shed some light on choosing the appropriate antifouling molecules in fabricating bioactive surfaces. ? 2021 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.