文献类型：期刊文献

英文题名：High nitrite accumulation in hydrogenotrophic denitrification at low temperature: Transcriptional regulation and microbial community succession

作者：Zhou, Jianmin[1];Ding, Lei[1];Cui, Changzheng[1];Lindeboom, Ralph E. F.[2]

机构：[1]East China Univ Sci & Technol, Natl Engn Res Ctr Ind Wastewater Detoxicat & Resou, Sch Resources & Environm Engn, Shanghai 200237, Peoples R China;[2]Delft Univ Technol, Dept Water Management, Sanit Engn Sect, NL-2628 CN Delft, Netherlands

年份：2024

卷号：263

外文期刊名：WATER RESEARCH

收录：;EI(收录号：20243116783110);WOS:【SCI-EXPANDED(收录号:WOS:001285144800001)】；

基金：This work was supported by the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry (B100-C-1503) , and the National Natural Science Foundation of China (51008125) .

语种：英文

外文关键词：Hydrogenotrophic denitrification; Low temperature; Nitrite accumulation; Denitrifying gene expression; Paracoccus; Hydrogenophaga

摘要：High Pressure Hydrogenotrophic Denitrification (HPHD) provided a promising alternative for efficient and clean nitrate removal. In particular, the denitrification rates at low temperature could be compensated by elevated H2 partial pressure. However, nitrite reduction was strongly inhibited while nitrate reduction was barely affected at low temperature. In this study, the nitrate reduction gradually recovered under long-term low temperature stress, while nitrite accumulation increased from 0.1 to 41.0 mg N/L. The activities of the electron transport system (ETS), nitrate reductase (NAR), and nitrite reductase (NIR) decreased by 45.8 %, 27.3 %, and 39.3 %, respectively, as the temperature dropped from 30 degrees C to 15 degrees C. Real time quantitative PCR analysis revealed that the denitrifying gene expression rather than gene abundance regulated nitrogen biotransformation. The substantial nitrite accumulation was attributed to the significant up-regulation by 54.7 % of narG gene expression and downregulation by 73.7 % of nirS gene expression in hydrogenotrophic denitrifiers. In addition, the nirS-gene-bearing denitrifiers were more sensitive to low temperature compared to those bearing nirK gene. The dominant populations shifted from the genera Paracoccus to Hydrogenophaga under long-term low temperature stress. Overall, this study revealed the microbial mechanism of high nitrite accumulation in hydrogenotrophic denitrification at low temperature.