文献类型：期刊文献

英文题名：Efficient biosynthesis of 3-hydroxypropionic acid from ethanol in metabolically engineered Escherichia coli

作者：Lu, Juefeng[1];Wang, Yuying[1];Xu, Mingcheng[1];Fei, Qiang[2];Gu, Yang[3];Luo, Yuanchan[1];Wu, Hui[1,4,5]

机构：[1]East China Univ Sci & Technol, Sch Biotechnol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Xi An Jiao Tong Univ, Sch Chem Engn & Technol, Xian, Peoples R China;[3]Chinese Acad Sci, Shanghai Inst Plant Physiol & Ecol, CAS Ctr Excellence Mol Plant Sci, Key Lab Synthet Biol,State Key Lab Plant Carbon Ni, Shanghai, Peoples R China;[4]Shanghai Collaborat Innovat Ctr Biomfg Technol, 130 Meilong Rd, Shanghai 200237, Peoples R China;[5]China Natl Light Ind Council, Key Lab Biobased Mat Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2022

卷号：363

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20223712734592);WOS:【SCI-EXPANDED(收录号:WOS:000862258600004)】；

基金：We thank Professor Shu Quan from East China University of Scienceand Technology for kindly providing us with the strain, E. coli BL27. This work was supported by the National Key R & D Program of China (2021YFC2103500) , Science and Technology Commission of Shanghai Municipality (21DZ1209100) . Partially supported by Open Funding Project of the State Key Laboratory of Bioreactor Engineering.

语种：英文

外文关键词：Escherichia coli; Ethanol utilization; 3-hydroxypropionic acid; Metabolic engineering; Whole -cell biocatalysis

摘要：Engineering microbial cell factories to convert CO2-based feedstock into chemicals and fuels provide a feasible carbon-neutral route for the third-generation biorefineries. Ethanol became one of the major products of syngas fermentation by engineered acetogens. The key building block chemical 3-hydroxypropionic acid (3-HP) can be synthesized from ethanol by the malonyl-CoA pathway with CO2 fixation. In this study, the effect of two ethanol consumption pathways on 3-HP synthesis were studied as well as the effect of TCA cycle, gluconeogenesis pathway, and transhydrogenase. And the 3-HP synthesis pathway was also optimized. The engineered strain synthesized 1.66 g/L of 3-HP with a yield of 0.24 g/g. Furthermore, the titer and the yield of 3-HP increased to 13.17 g/L and 0.57 g/g in the whole-cell biocatalysis system. This study indicated that ethanol as feedstock had the potential to synthesize 3-HP, which provided an alternative route for future biorefinery.