文献类型：期刊文献

英文题名：Metabolic profiling and flux distributions reveal a key role of acetyl-CoA in sophorolipid synthesis by Candida bombicola

作者：Yang, Linbing[1];Li, Youyuan[1];Zhang, Xinkai[1];Liu, Ting[1];Chen, Jun[1];Wei, Liujing[1];Hua, Qiang[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg Technol, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2019

卷号：145

起止页码：74

外文期刊名：BIOCHEMICAL ENGINEERING JOURNAL

收录：;EI(收录号：20190806538510);WOS:【SCI-EXPANDED(收录号:WOS:000466054400009)】；

基金：This study was supported by National Special Fund for State Key Laboratory of Bioreactor Engineering (2060204) and Fundamental Research Funds for the Central Universities (222201714052).

语种：英文

外文关键词：Sophorolipid; Metabolic flux; Metabolic profile; Acetyl-CoA; Candida bombicola; Biosurfactant

摘要：Sophorolipids are important biosurfactants produced by the wild-type strain of Candida bombicola ATCC 22214, with antimicrobial, antiviral, and anticancer activities. Our previous study showed that a mutant strain lacking multifunctional enzyme type 2 displayed extremely low sophorolipid production capacity compared to the wild type strain. In the present study, we aimed to reveal the possible reasons for this deficiency through a series of analytical methods. First, comparative metabolic profiling analysis between the wild-type and mutant strains was performed. Multivariate statistical analysis, including principal component analysis and orthogonal partial least squares discriminant analysis, showed that nine potential metabolites were closely associated with the metabolic differences between strains. Second, metabolic flux analysis revealed that an inadequate supply of intracellular acetyl-CoA in the mutant strain could significantly affect the biosynthesis of sophorolipids. In addition, supplementation of citric acid recovered the sophorolipid production in the mutant strain by approximately 83%, suggesting that citrate metabolism plays an important role in sophorolipid biosynthesis. These findings provide novel theoretical insights for further improving the production of sophorolipids in C. bombicola.