文献类型：期刊文献

英文题名：Practical Assay for Nitrite and Nitrosothiol as an Alternative to the Griess Assay or the 2,3-Diaminonaphthalene Assay

作者：Shen, Yanming[1];Zhang, Quanjuan[1];Qian, Xuhong[1];Yang, Youjun[1]

机构：[1]E China Univ Sci & Technol, Shanghai Key Lab Chem Biol, Sch Pharm, Shanghai 200237, Peoples R China

年份：2015

卷号：87

期号：2

起止页码：1274

外文期刊名：ANALYTICAL CHEMISTRY

收录：;EI(收录号：20150600505633);WOS:【SCI-EXPANDED(收录号:WOS:000348332300061)】；

基金：We thank Ms. Linlin Song for assistance with pH titration and selectivity studies. The work is supported by Shanghai Rising-Star Program (No. 13QA1401200), Doctoral Fund of Ministry of Education of China (No. 20110074120008), and the National Natural Science Foundation of China (Nos. 21106043 and 21372080).

语种：英文

外文关键词：Reaction kinetics - Disease control - Water quality - Nitric oxide - Probes - Fluorescence - Absorption spectroscopy

摘要：Nitrite is a heavily assayed substrate in the fields of food safety, water quality control, disease diagnosis, and forensic investigation and more recently in basic biological studies on nitric oxide physiology and pathology. The colorimetric Griess assay and the fluorimetric 2,3-diaminonaphthalene (DAN) assay are the current gold standards for nitrite quantification. They are not without limitations, yet have amazingly survived 156 and 44 years, respectively, due to the lack of a practical alternative. Both assays exhibit slow detection kinetics due to inactivation of nucleophiles under strongly acidic media, require an extensive incubation time for reaction to go completion, and hence offer a limited detection throughput. By converting an intermolecular reaction of the Griess assay intramolecularly, we designed a novel probe (NT555) for nitrite detection, which displays superior detection kinetics and sensitivity. NT555 was constructed following our covalent-assembly probe design principle. Upon detection, it affords a gigantic bathochromic shift of the absorption spectrum and a sensitive turn-on fluorescence signal from a zero background, both of which are typical of an assembly type probe. Overall, NT555 has addressed various difficulties associated with the Griess and the DAN assays and represents an attractive alternative for practical applications.