文献类型：期刊文献

英文题名：Application of improved top-down approach in maximizing CHO cell mass and productivity in fed-batch culture

作者：Sun, Ya-Ting[1];Zhao, Liang[1];Liu, Xu-Ping[1];Hu, Dong-dong[1];Fan, Li[1];Tan, Wen-Song[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2013

卷号：88

期号：7

起止页码：1237

外文期刊名：JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY

收录：;EI(收录号：20132616453593);WOS:【SCI-EXPANDED(收录号:WOS:000320038700007)】；

基金：This work was supported by the National Natural Science Foundation of China (No. 21106045), the National High Technology Research and Development Program of China (863 Program) (No. 2010AA022905, No. 2012AA02A303), and Shanghai University Funding Scheme for Young Teacher Training (No. hlg11011).

语种：英文

外文关键词：CHO cells; top-down approach; fed-batch culture; monoclonal antibody; amino acids

摘要：Background Enhancing the efficiency of process development is a key issue for the production of recombinant therapeutic protein. To maximize both cell mass and productivity with limited time and effort, an improved top-down approach was adopted in the development and optimization of fed-batch culture. Results Two-round optimization was carried out to refine the nutrients following an investigation into the effect of complete feeding medium. After the first round of optimization, cell growth was improved with the integral of viable cell concentration increased by 32.2%. After the second round of optimization, cell productivity was improved with the specific antibody production rate increased 1.5-fold, and cell longevity was also prolonged using improved feeding strategy. In the optimized fed-batch culture in a 2 L bioreactor, the maximum cell concentration was increased by 68% and final antibody titer was increased 3.9-fold compared with the fed-batch culture with simple feeding medium. Conclusion The improved top-down approach helps to reveal the interaction of different nutrients and their important roles in the improvement of cell proliferation and production. Thus, the common defects of a top-down approach can be avoided. This approach is practicable and satisfactory for efficient development of a high-yielding process. (c) 2013 Society of Chemical Industry