文献类型：期刊文献

英文题名：HPLC determination of anethole trithione and its application to pharmacokinetics in rabbits

作者：Jing, Qiufang; Shen, Yongjia; Ren, Fuzheng; Chen, Jianming; Jiang, Zhiting; Peng, Baoliang; Leng, Yixin; Dong, Jing

机构：[1]E China Univ Sci & Technol, Sch Chem & Pharmaceut, Dept Pharmaceut Engn, Shanghai 200237, Peoples R China;[2]Second Mil Med Univ, Sch Pharm, Dept Pharmaceut, Shanghai 200433, Peoples R China

年份：2006

卷号：42

期号：5

起止页码：613

外文期刊名：JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000242651700013)】；

语种：英文

外文关键词：anetbole trithione; tablets; self-microemulsifying drug delivery system; bioavailability; HPLC

摘要：To evaluate the relative bioavailability of anethole trithione (ATT) from self-microemulsifying drug delivery system (SMEDDS) and tablet, a sensitive, accurate and reliable liquid chromatography method was developed and validated to determine ATT in rabbit plasma. Chromatographic separation was performed on a Diamonsil C-18 column by using a mixture of methanol-water (90:10, v/v) delivered at a flow rate of 1.0 ml/min. The wavelength was set at 348 nm and mifepristone was used as the internal standard. A linear relationship for ATT was found in the range of 0.5-32 ng/ml. The mean extraction recoveries of ATT determined over three concentrations were 84.7 +/- 5.8, 92.3 +/- 3.4 and 89.9 +/- 5.1%. After administration of SMEDDS and tablets to rabbits, significant differences were found in main pharmacokinetic parameters of T-max, C-max and AUC(0-infinity) between these two formulations, and a 2.5-fold enhancement of relative bioavailability of ATT was observed from the SMEDDS compared with tablets. (c) 2006 Elsevier B.V. All rights reserved.