文献类型：期刊文献

英文题名：Determination of caffeoylquinic acid derivatives in Azolla imbricata by chitosan-based matrix solid-phase dispersion coupled with HPLC-PDA

作者：Wu, Weilin[1];Qian, Wenqi[2];Hao, Haitian[1];Kang, Yun[2];Wang, Yaqin[2];Deng, Yuanrong[3];Ni, Chengjie[3];Huang, Jianming[2];Weng, Weiyu[1]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Dept Pharmaceut, Shanghai 200237, Peoples R China;[2]Fudan Univ, Sch Pharm, Dept Pharmacognosy, Shanghai 201203, Peoples R China;[3]Fujian Joan Pharmaceut Co Ltd, Fuzhou 350100, Fujian, Peoples R China

年份：2019

卷号：163

起止页码：197

外文期刊名：JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000451360900024)】；

语种：英文

外文关键词：Azolla imbricata; Matrix solid-phase dispersion; Caffeoylquinic acid; HPLC

摘要：Five main components of Azolla imbricata were isolated and identified as caffeoylquinic acid derivatives, four of which were isolated from this species for the first time. The five compounds exhibited strong antioxidant activities and were used as chemical markers for quantitative analysis. A chitosan-based matrix solid-phase dispersion (MSPD) extraction coupled with high-performance liquid chromatography (HPLC) was developed for the determination of the five analytes in A. imbricata. The optimal conditions for the chitosan-based MSPD extraction were as follows: low viscosity chitosan HL-1 as the dispersant, sample-to-dispersant mass ratio of 1:1, 10 mL of a methanol-sulfuric acid aqueous solution (0.2 M) (7:3, v/v) as the elution solvent, and a grinding time of 1 min. Compared with ultrasonic assisted extraction (UAE), the chitosan-based MSPD extraction exhibited higher extraction efficiency, consumed less solvent and time, and provided a cleaner extract. Compared with C-18-based MSPD, the developed method was less expensive and more environmentally-friendly. The validated MSPD-HPLC method was efficient, reliable, and applicable to the quantification of caffeoylquinic acids in A. imbricata. (C) 2018 Elsevier B.V. All rights reserved.