文献类型：期刊文献

英文题名：Doxorubicin-loaded redox-responsive amphiphilic dendritic porphyrin conjugates for chemotherapy and photodynamic therapy

作者：Liu, Feng[1];Zhang, Yang[1];Pan, Xiuwei[1];Xu, Lei[1];Xue, Yudong[1];Zhang, Weian[1]

机构：[1]E China Univ Sci & Technol, Coll Mat Sci & Engn, Shanghai Key Lab Funct Mat Chem, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2016

卷号：6

期号：62

起止页码：S7552

外文期刊名：RSC ADVANCES

收录：;EI(收录号：20162702556505);WOS:【SCI-EXPANDED(收录号:WOS:000378755200106)】；

基金：This work was financially supported by the National Natural Science Foundation of China (No. 21574039), Research Innovation Program of SMEC (No. 14ZZ065) and Shanghai Pujiang Program under 14PJ1402600. W. Z. also acknowledges the support from the Fundamental Research Funds for the Central Universities.

语种：英文

外文关键词：Chemotherapy - Photodynamic therapy - Solutions - Targeted drug delivery - Photosensitizers - Cell proliferation - Sulfur compounds - Controlled drug delivery - Micelles - Polyethylene oxides - Polyethylene glycols

摘要：Combination therapy has gained enormous attention due to its high effectiveness in killing cancer cells. Herein, a novel reduction-responsive drug carrier based on amphiphilic dendritic copolymer was constructed for the combination treatment of chemotherapy with photodynamic therapy (PDT). The porphyrin (TPP) disulfide coupled third-generation dendronized poly(ethylene glycol) (PEG-G3-OH) copolymer (TPP-S-S-G3) was synthesized via disulfide linkages between dendritic polyester and porphyrin photosensitizers, which could further self-assemble into spherical micelles in aqueous solution. Compared to the linear TPP-S-S-G0 copolymer, the dendritic TPP-S-S-G3 copolymer could have a higher drug loading content and efficiency. The uptake of doxorubicin (DOX)-loaded TPP-S-S-G3 micelles by MCF-7 cells and intracellular release of porphyrin and DOX was investigated by flow cytometry and CLSM, respectively, and the results exhibited a fast uptake of TPP-S-S-G3 micelles and drug release. Cytotoxicity assay of DOX-loaded TPP-S-S-G3 micelles were evaluated by MTT assay. It indicated that DOX-loaded TPP-S-S-G3 micelles exhibited a higher cellular proliferation inhibition than free DOX, free porphyrin and DOX-loaded linear TPP-S-S-G0 micelles under light irradiation. Thus, the reduction-responsive dendritic copolymer presented the great potential in the combination therapy between chemotherapy and PDT.