文献类型：期刊文献

英文题名：Electrocatalytic Oxidation of Glucose by the Glucose Oxidase Immobilized in Graphene-Au-Nafion Biocomposite

作者：Zhou, Kangfu[1];Zhu, Yihua[1];Yang, Xiaoling[1];Li, Chunzhong[1]

机构：[1]E China Univ Sci & Technol, Sch Mat Sci & Engn, Minist Educ, Key Lab Ultrafine Mat, Shanghai 200237, Peoples R China

年份：2010

卷号：22

期号：3

起止页码：259

外文期刊名：ELECTROANALYSIS

收录：;EI(收录号：20231713975478);WOS:【SCI-EXPANDED(收录号:WOS:000274538400001)】；

基金：This work was supported by the National Natural Science Foundation of China (20676038), the Key Project of Science and Technology for Ministry of Eductaion (107045), the Innovation Program of Shanghai Municipal Education Commission (09ZZ58). the Program of Shanghai Subject Chief Scientist (OSXDI.401500), the Shuguang ScholarTracking Foundation of Shanghai (08GG09), and the Shanghai Leading Academic Discipline Project (project number: B502).

语种：英文

外文关键词：Glucose biosensor; Graphene; Au nanoparticles; Electrocatalytic oxidation; Synergistic effect; Biosensors; Nanoparticles

摘要：Graphene was successfully prepared and well separated to individual sheets by introducing -SO3-. XRD and TEM were employed to characterize the graphene. UV-visible absorption spectra indicated that glucose oxidase (GOx) could keep bioactivity well in the grapene-Au biocomposite. To construct a novel glucose biosensor, graphene, Au and GOx were co-immobilized in Nafion to further modify a glassy carbon electrode (GCE). Electrochemical measurements were carried out to investigate the catalytic performance of the proposed biosensor. Cyclic voltammograms (CV) showed the biosensor had a typical catalytic oxidation response to glucose. At the applied potential +0.4 V, the biosensor responded rapidly upon the addition of glucose and reached the steady state current in 5 s, with the present of hydroquinone. The linear range is from 15 mu M to 5.8 mM, with a detection limit 5 mu M (based oil the S/N=3). The Michaelis-Menten constant was calculated to be 4.4 mM according to Lineweaver-Burk equation. In addition, the biosensor exhibits good reproducibility and long-term stability. Such impressive properties could be ascribed to the synergistic effect of graphenc-Au integration and good biocompatibility of the hybrid material.