文献类型：期刊文献

英文题名：Dominance of Pseudomonas in bacterial community and inhibition of fumarate addition pathway by injection of nutrients in oil reservoir revealed by functional gene and their transcript analyses

作者：Liu, Jin-Feng[1,2,6];Lu, Yu-Wei[1,2,6];Liu, Xiao-Bo[3];Li, Bai-Guang[3];Sun, Yu-Fei[4];Zhou, Lei[1,2,6];Liu, Yi-Fan[1,2,6];Yang, Shi-Zhong[1,2,6];Gu, Ji-Dong[5];Mu, Bo-Zhong[1,2,6]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[3]PetroChina, Daqing Oilfield Co Ltd, Oil Prod Factory 2, Daqing 163414, Heilongjiang, Peoples R China;[4]PetroChina, Daqing Oilfield Co Ltd, Oil Prod Factory 4, Daqing 163511, Heilongjiang, Peoples R China;[5]Univ Hong Kong, Sch Biol Sci, Pokfulam Rd, Hong Kong, Peoples R China;[6]East China Univ Sci & Technol, Engn Res Ctr Microbial Enhanced Oil Recovery, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2020

卷号：153

外文期刊名：INTERNATIONAL BIODETERIORATION & BIODEGRADATION

收录：;EI(收录号：20202708899259);WOS:【SCI-EXPANDED(收录号:WOS:000564599100005)】；

基金：We are grateful to the management of Daqing Oil Field for sampling support. This work was supported by the National Science Foundation of China (41673084, 41530318), the Shanghai International Collaboration Program (18230743300).

语种：英文

外文关键词：Oil reservoir; Microbial composition; Functional gene; Transcripts; Nutrient injection; Evolution

摘要：The microbial community composition in subsurface oil reservoirs is responsible for the biogeochemical reactions of hydrocarbon transformation. In this study, the composition, major functions and gene expression activity of the microorganisms in production water from an oil reservoir were analyzed prior to and after injection nutrients using high-throughput 16S rRNA gene sequencing, functional gene and their transcript profiling. Pseudomonas spp. was the dominant bacteria showing a quick increase in the bacterial population. The injection of nutrients lowered the activity of assA-bearing bacteria, but accelerated the activity of methanogens. The results show that the anaerobic hydrocarbon degradation was actively occurring in situ oil reservoir sampled, Smithella was the active anaerobic hydrocarbon degrading bacteria, and Methanoculleus and Methanothrix were the actively dominant methanogens. The present study provides a snapshot of the practical technology with insightful results to support the research, development and utilization of reservoir through biological augmentation.