文献类型：期刊文献

英文题名：High-density heterotrophic cultivation of a cell-wall-deficient Chlamydomonas reinhardtii strain by fed-batch strategy

作者：Zhou, Kaidi[1];Yu, Tao[1];Wan, Minxi[1];Bao, Zhengxu[2];Bai, Wenming[2];Wang, Weiliang[1];Li, Yuanguang[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Mail Box 301,Meilong Rd 130, Shanghai 200237, Peoples R China;[2]Baoshan Zeyuan Algae Hlth Technol Co Ltd, Baoshan 678208, Peoples R China

年份：2025

卷号：47

期号：4

外文期刊名：BIOTECHNOLOGY LETTERS

收录：;EI(收录号：20252818752795);WOS:【SCI-EXPANDED(收录号:WOS:001527898100001)】；

基金：This work was supported by Baoshan Zeyuan Algae Health Technology Co., Ltd.

语种：英文

外文关键词：Cell-wall-deficient Chlamydomonas reinhardtii; Heterotrophic cultivation; Fed-batch cultivation; Process optimization

摘要：Chlamydomonas reinhardtii CW15, a cell-wall-deficient strain, is a widely used chassis in microalgal genetic engineering. However, its low cell density under autotrophic and mixotrophic cultivation restricts industrial scalability. This study aimed to establish a cost-effective, high-density heterotrophic cultivation process for C. reinhardtii CW15. Initially, an optimized AP medium (Tris-free TAP with K2HPO4 as the sole phosphorus source) significantly reduced medium costs while achieving a cell density of 0.446 g/L. Subsequently, a fed-batch process in a 5 L fermentor yielded a record cell density of 22.1 g/L (growth rate: 94.6 mg/Lh) at 233 h by optimizing nutrient supplementation. Scaling up to a 50 L fermentor revealed challenges in ammonia volatilization and salinity accumulation during sterilization. These were resolved by substituting the nitrogen source with ammonia water and supplementing ammonium acetate as a dual nitrogen and partial carbon source, ultimately achieving 30.2 g/L (140 mg/Lh) after 215 h-the highest reported density for cell-wall-deficient C. reinhardtii. This work provides an efficient and scalable cultivation strategy, facilitating its industrial and biotechnological applications.