文献类型：期刊文献

中文题名：重组工业红色糖多孢菌优化红霉素发酵液组分

英文题名：Improvement of erythromycin A purity and production in a recombinant industrial Saccharopolyspora erythraea strain

作者：李学志[1];钱江潮[1];陈云[1];杭海峰[1];储炬[1];庄英萍[1];张嗣良[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2009

卷号：34

期号：3

起止页码：152

中文期刊名：中国抗生素杂志

外文期刊名：Chinese Journal of Antibiotics

收录：CSTPCD;;Scopus;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：863工业生物技术重点项目(2006AA020304);上海市科委重点项目(074319115-3)

语种：中文

中文关键词：红霉素;组分;重组菌;发酵

外文关键词：Erythromycin; Components; Recombinant; Fermentation

摘要：本文对代谢工程改造红色糖多孢菌ZL1004进行了摇瓶发酵试验,摇瓶发酵结束时重组菌红霉素生物效价与工业生产菌HL3168相比提高20%,红霉素A组分比例由84%提高到94%,红霉素A浓度3.98mg/ml,相比出发菌3.25mg/ml提高22%,重组菌中红霉素B、C与出发菌相比分别减少了51%和58%,实现了红霉素组分的优化。对重组菌摇瓶发酵过程生理代谢进行了初步的研究,表明重组菌生理代谢特性与原出发菌没有明显差异。对重组菌发酵过程中红霉素组分的变化规律进行分析,发现红霉素A、B、C组分的变化规律与eryK和eryG基因转录水平能较好地对应。
In this paper, the performance of recombinant industrial Saccharopolyspora erythraea strain ZL1004 was studied in shake flasks. The ratio of Er-A was increased from 84% to 94% with a final concentration of 3.98mg/ml which was 22% higher than that of the original HL3168 strain （3.25mg/ml）. Furthermore, the concentrations of Er-B and Er-C were decreased significantly by 51% and 58% respectively. The study on the primary physiological characteristics of recombinant strain ZL1004 showed no apparent difference in glucose, nitrogen, phosphoric consumption rate in comparison with HL3168 strain. The analysis of Er-A, Er-B and Er-C ratios of both ZL1004 and HL3168 in the culture broth during the fermentation process showed a good correlation with the result of transcription level of the eryK and eryG genes in these strains.