文献类型：期刊文献

英文题名：Multiple-fingerprint analysis of Poria cocos polysaccharide by HPLC combined with chemometrics methods

作者：Zhang, Yilin[1];Wu, Mengqi[1];Xi, Jingrui[1];Pan, Chen[2];Xu, Zhizhen[1];Xia, Wei[1];Zhang, Wenqing[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]Natl Med Prod Adm, Shanghai Inst Food & Drug Control, Key Lab Monitoring & Evaluat Cosmet, Shanghai 201203, Peoples R China

年份：2021

卷号：198

外文期刊名：JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000636654000024)】；

语种：英文

外文关键词：Multiple fingerprints; Poria cocos polysaccharides; Chemometric analysis; Quality evaluation; HPLC

摘要：In this study, the multiple fingerprints, which were integrated with HPGFC-ELSD (high performance gel filtration chromatography - evaporative light scattering detector) fingerprint, PMP-HPLC-DAD (1-phenyl-3-methyl-5-pyrazolone-high performance liquid chromatography - diode array detector) fingerprint of complete acid hydrolysates and HILIC-HPLC-ELSD (hydrophilic interaction - high performance liquid chromatography - evaporative light scattering detector) fingerprint of enzyme hydrolysates, were established to evaluate the quality of polysaccharides from Poria cocos (PCPs). The similarity evaluation showed that 16 batches of PCPs from different origins had high similarity in structural characteristics based on the multiple fingerprints. The chromatographic data of multiple fingerprints of PCPs were fused, processed and analyzed by chemometric methods including HCA (hierarchical cluster analysis), PCA (principal component analysis) and PLS-DA (partial least squares discriminant analysis). The 16 batches of PCPs were divided into 3 categories in PCA, indicating a certain relationship between the structural characteristics and the origins. PLS-DA analysis indicated that Man, Glc, Gal, Fuc, the components with m/z of 2.22 x 10(4)similar to 1.53 x 10(4) Da and 3.46 x 10(3)similar to 2.69 x 10(3) Da, oligosaccharides with DPs of 6 and 7, respectively, could be regarded as potential chemical markers for the classification of PCPs from different origins. According to the multiple fingerprints and chemometric analysis, the two commercial samples were proved to be adulterants. (C) 2021 Elsevier B.V. All rights reserved.