文献类型：会议论文

英文题名：Bienzymatic glucose biosensor based on co-immobilization of glucose oxidase and horseradish peroxidase on gold nanoparticles-mesoporous silica matrix

作者：Xu, Lihuan[1];Zhu, Yihua[1];Li, Yaoxia[1];Yang, Xiaoling[1];Li, Chunzhong[1]

机构：[1]E China Univ Sci & Technol, Sch Mat Sci & Engn, Key Lab Ultrafine Mat, Minist Educ, Shanghai 200237, Peoples R China

会议论文集：2nd IEEE International Nanoelectronics Conference

会议日期：MAR 24-27, 2008

会议地点：Shanghai, PEOPLES R CHINA

语种：英文

外文关键词：Bienzymatic biosensor; Glucose oxidase; Horseradish peroxidase; Mesoporous silica; Gold nanoparticles

摘要：A newly blenzymatic glucose biosensor was prepared for selective and sensitive detection of glucose. This biosensor was built by simultaneous immobilization of glucose oxidase (GOx) and horseradish peroxidase (HRP) into the gold nanoparticles-mesoporous silica composite (GN-Ps-WS) matrix, and then casting the composite on glassy carbon electrode (C.CE) using Naflon as a cross-Unker. Cyclic voltammetry (CV) and amperometry were employed to investigate the catalytic behavior of the biosensor to the oxidation of glucose. As a result, the biosensor exhibited an excellent bioelectrocatalytic response to glucose, as well as good long-term stability and reproducibility.