文献类型：期刊文献

中文题名：粘质沙雷氏菌利用蔗糖和柠檬酸铵生产2,3-丁二醇的研究

英文题名：Study on 2,3-Butanediol Production by Serratia Marcescens Using Sucrose and Ammonium Citrate

作者：杨云龙[1];张燎原[1];孙建安[1];魏东芝[1];周文瑜[1];沈亚领[1];朱家文[2]

机构：[1]华东理工大学生物反应器工程国家重点实验室鲁华生物技术研究所,上海200237;[2]华东理工大学化工分离研究所,上海200237

年份：2009

卷号：26

期号：9

起止页码：41

中文期刊名：化学与生物工程

外文期刊名：Chemistry & Bioengineering

收录：CSTPCD

基金：国家863计划资助项目(2006AA02Z243);国家重点实验室专项经费资助项目(2060204);上海市重点学科建设资助项目(B505)

语种：中文

中文关键词：粘质沙雷氏菌;无机氮源;2,3-丁二醇;响应面法;分批补料发酵

外文关键词：Serratia marcescens;inorganic nitrogen source; 2,3-butanediol; response surface methodology;fed-bath fermentation

摘要：研究了几种无机氮源对粘质沙雷氏菌发酵生物量和产物2,3-丁二醇形成的影响,在确定柠檬酸铵为无机氮源的基础上,利用响应面法(RSM)对柠檬酸铵和硫酸锰的浓度进行了优化,得出了最佳浓度,并以此最优成分进行了摇瓶发酵和分批补料发酵。在摇瓶发酵中,110g·L-1的蔗糖最终被转化成44g·L-1的2,3-丁二醇,转化率为0.4g.g-1,产率为1.13g·L-1.h-1;在分批补料发酵中,共有166g·L-1的蔗糖被消耗,2,3-丁二醇的最高浓度为81.2g.L-1,乙偶姻的浓度为7.7g·L-1,2,3-丁二醇转化率达到0.489g.g-1,产率达到1.7g·L-1.h-1。
Effects of several inorganic nitrogen sources on biomass and 2,3-butanediol production by Serratia marcescens were studied. The results showed that ammonium citrate was the optimum inorganic nitrogen source. Based on the previous experiments, ammonium citrate and manganese sulfate were studied by response surface methodology to determine the optimized concentration. The optimal medium was used to perform sha- king fermentation and fed-batch fermentation experiments. Ultimately, the strain converted 110 g ·L^-1 sucrose to 44 g ·L^-1 2,3-butanediol, and the transformation efficiency and productivity were 0. 4 g ·g^-1 and 1.13 g ·L^-1 ·h^-1respectively in shaking flasks. In fed-batch fermentation, a 2,3-butanediol level of 81.2 g ·L^-1 and acetoin level of 7.7 g ·L^-1 were obtained from 166 g ·L^-1 sucrose, and the transformation efficiency and productivity of 2,3-butanediol achieved to O. 489 g ·g^-1 and 1.7 g ·L^-1 ·h^-1 ; respectively.