文献类型：期刊文献

英文题名：Highly selective and wash-free visualization of resistant bacteria with a relebactam-derived fluorogenic probe

作者：Chen, Yefeng[1];Xu, Minqiu[1];Xu, Weipan[1];Song, Heng[1];Hu, Liqiang[1];Xue, Shuyuan[1];Zhang, Shuangzhan[1];Qian, Xiana[1];Xie, Hexin[1]

机构：[1]East China Univ Sci & Technol, Sch Pharm, State Key Lab Bioreactor Engn, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China

年份：2019

卷号：55

期号：67

起止页码：9919

外文期刊名：CHEMICAL COMMUNICATIONS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000481528300030)】；

基金：This work was financially supported by NSFC-BRICS (81861148020), NSFC (21472048), and the Research Program of State Key Laboratory of Bioreactor Engineering. We thank Ms Yuanyuan Su from Leica Microsystems Trading Ltd. Shanghai for technical support.

语种：英文

摘要：Reported herein is a relebactam-derived fluorogenic reagent for covalent labeling of serine beta-lactamases (SBLs), which are the major causes of bacterial resistance to beta-lactam antibiotics. This highly selective imaging reagent generates over 300-fold stronger near-infrared fluorescence signals upon covalently bonding to SBLs, allowing wash-free visualization of live antimicrobial-resistant bacteria.