文献类型：期刊文献

英文题名：Multi-tissue scRNA-seq reveals immune cell landscape of turbot (Scophthalmus maximus)

作者：Chen, Weijie[1];Huang, Jianchang[1];Wang, Wei[1];Wang, Ying[1];Chen, Hao[1];Wang, Qiyao[1];Zhang, Yuanxing[2,4];Liu, Qin[1,3];Yang, Dahai[1,4]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Southern Marine Sci & Engn Guangdong Lab Zhuhai, Zhuhai 519000, Peoples R China;[3]Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China;[4]Shanghai Engn Res Ctr Maricultured Anim Vaccines, Shanghai 200237, Peoples R China

年份：2022

卷号：2

期号：4

起止页码：550

外文期刊名：FUNDAMENTAL RESEARCH

收录：WOS:【ESCI(收录号:WOS:000855578400001)】；

语种：英文

外文关键词：ScRNA-seq; Fish immune cell landscape; Dynamic immune cell responses; Edwardsiella piscicida infection; Turbot

摘要：In vertebrates, bony fishes possess not only innate immune cells but also T and B cells that are equivalent to those in mammals. However, the precise sub-cluster of immune cells in teleost fish remains largely unknown. Herein, we developed a dynamic bacterial infection model in turbot (Scophthalmus maximus) and created a fish immune cell landscape (FICL) for a primary lymphoid organ (head kidney), a secondary lymphoid organ (spleen), and barrier tissues (gills and posterior intestine). Moreover, through comprehensive characterization of the expression profiles of 16 clusters, including dendritic cells-like (DCs-like), macrophages (M Phi s), neutrophils, NK cells, as well as 12 sub-clusters of T and B cells, we found that CD8(+) CTLs, CD4(-) CD8(-) T, Th17 and ILC3-2 like cells possess a bifunctional role associated with cytotoxicity and immunoregulation during bacterial infection. To our knowledge, these results could provide a useful resource for a better understanding of immune cells in teleost fish and could act as a comprehensive knowledge base for assessing the evolutionary mechanism of adaptive immunity in vertebrates.