文献类型：期刊文献

英文题名：Determination of nucleosides in serum by two-dimensional magnetic solid-phase extraction microfluidic chip/liquid chromatography-mass spectrometry

作者：Cheng, Yongzhe[1];Sun, Yangkun[1];Wang, Ningning[1];Jia, Qiangqiang[2];Zhao, Linghao[1];Wu, Zeyu[1];Wang, Yuerong[1];Zhang, Hongyang[1];Zhang, Min[3];Hu, Ping[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]Qinghai Univ, Coll Pharm, Xining 810016, Peoples R China;[3]East China Univ Sci & Technol, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met, Sch Pharm, Shanghai 200237, Peoples R China

年份：2026

卷号：1768

外文期刊名：JOURNAL OF CHROMATOGRAPHY A

收录：;EI(收录号：20260219871817);WOS:【SCI-EXPANDED(收录号:WOS:001668979900001)】；

基金：This work was supported by the National Natural Science Foundation

语种：英文

外文关键词：Microfluidic chip; Two-dimensional magnetic solid phase; extraction; Liquid chromatography-mass spectrometry; Magnetic nanomaterials; Nucleosides

摘要：A two-dimensional magnetic solid-phase extraction microfluidic chip (2D-MSPE-chip) was designed in this paper. A microcolumn array was integrated before two serially connected extraction chambers to filter particles in the sample. The two magnetic nanomaterials with orthogonal separation mechanism of Fe3O4@UiO-66-C18 and Fe3O4@MOF@COF-B(OH)(2) were filled into the extraction chamber, respectively, and were used to effectively eliminate matrix effects and enrich the targets. The 2D-MSPE-chip was combined with liquid chromatography-mass spectrometry (LC-MS) for the determination of eight nucleosides in serum samples. The validation results showed that the 2D-MSPE-chip/LC-MS method had excellent linear range (2-1000 ng/mL), high sensitivity (detection limit of 0.02-0.22 ng/mL), good repeatability (intra-day precision <= 5.2%, inter-day precision <= 7.2%) and a negligible absolute matrix effect (93.1%-101.9%). The recovery rate of the spiked serum samples of prostate cancer patients and healthy person was 90.8%-109.1%, indicating a high accuracy. The inosine level decreased and adenosine level increased in the serum of prostate cancer patients (p < 0.01), suggesting the correlation between nucleosides and the cancer.