文献类型：期刊文献

英文题名：Preparation, Characterization, In Vitro Bioactivity, and Cellular Responses to a Polyetheretherketone Bioactive Composite Containing Nanocalcium Silicate for Bone Repair

作者：Ma, Rui[1];Tang, Songchao[2];Tan, Honglue[1];Qian, Jun[2];Lin, Wentao[1];Wang, Yugang[1];Liu, Changsheng[2];Wei, Jie[2];Tang, Tingting[1]

机构：[1]Shanghai Jiao Tong Univ, Sch Med, Shanghai Peoples Hosp 9, Shanghai Key Lab Orthoped Implants,Dept Orthoped, Shanghai 200011, Peoples R China;[2]E China Univ Sci & Technol, Minist Educ, Key Lab Ultrafine Mat, Shanghai 200237, Peoples R China

年份：2014

卷号：6

期号：15

起止页码：12214

外文期刊名：ACS APPLIED MATERIALS & INTERFACES

收录：;EI(收录号：20143518096558);WOS:【SCI-EXPANDED(收录号:WOS:000340446300049)】；

基金：This research was financially supported by the National Natural Science Foundation of China (Nos. 31271015, 81271705, and 51173041) and the Shanghai Science and Technology Development Fund (Nos. 13DZ2294000, 13JC1403900, 12nm0500400, and 12441903600).

语种：英文

外文关键词：polyetheretherketone; calcium silicate; composite; bioactivity; cellular responses

摘要：In this study, a nanocalcium silicate (n-CS)/polyetheretherketone (PEEK) bioactive composite was prepared using a process of compounding and injection-molding. The mechanical properties, hydrophilicity, and in vitro bioactivity of the composite, as well as the cellular responses of MC3T3-E1 cells (attachment, proliferation, spreading, and differentiation) to the composite, were investigated. The results showed that the mechanical properties and hydrophilicity of the composites were significantly improved by the addition of n-CS to PEEK. In addition, an apatite-layer formed on the composite surface after immersion in simulated body fluid (SBF) for 7 days. In cell culture tests, the results revealed that the n-CS/PEEK composite significantly promoted cell attachment, proliferation, and spreading compared with PEEK or ultrahigh molecular weight polyethylene (UHMWPE). Moreover, cells grown on the composite exhibited higher alkaline phosphatase (ALP) activity, more calcium nodule-formation, and higher expression levels of osteogenic differentiation-related genes than cells grown on PEEK or UHMWPE. These results indicated that the incorporation of n-CS to PEEK could greatly improve the bioactivity and biocompatibility of the composite. Thus, the n-CS/PEEK composite may be a promising bone repair material for use in orthopedic clinics.