文献类型：期刊文献

英文题名：Quorum Sensing-Based Detection and Biodegradation of Phthalate Esters by Engineered Escherichia coli and Pseudomonas aeruginosa

作者：Nina, Mario Roque Huanca[1];Zhang, Xiaoyan[1];Fan, Daidi[2];Bai, Yunpeng[1,2]

机构：[1]East China Univ Sci & Technol, Shanghai Collaborat Innovat Ctr Biomfg, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Northwest Univ, Shaanxi R&D Ctr Biomat & Fermentat Engn, Sch Chem Engn, Xian 710069, Shaanxi, Peoples R China

年份：2025

卷号：73

期号：44

起止页码：27990

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20254519452113);WOS:【SCI-EXPANDED(收录号:WOS:001600323800001)】；

基金：This work was financially sponsored by the National Key R&D Program of China (grant nos. 2023YFA0913600) and the National Natural Science Foundation of China (grant nos. 22378120).

语种：英文

外文关键词：autonomous biodegradation; enzyme catalysis; protein engineering; synthetic biology; whole-cellbiosensor

摘要：Phthalate esters (PAEs) are widely used in industry and have become prevalent toxic contaminants. Microbial biodegradation is promising, but its application is limited by the lack of efficient strains that can degrade a broad spectrum of PAEs. In this study, protein evolution was employed to generate a new variant of a PAE-degrading enzyme, GoEst15V4, which exhibited 2.5- to 15-fold higher specific activity than the wild-type enzyme against p-nitrophenyl butyrate (pNPB) and nine other PAEs, including bulky substrates such as di(2-ethylhexyl) phthalate (DEHP), dihexyl phthalate, and diphenyl phthalate (DPHP). Additionally, autonomous whole-cell biodegraders containing a gene circuit XylS-QS-GV4 (XylS(PA)-LuxI-LuxR-GoEst15V4) were constructed, and the new engineered enzyme was expressed autonomously in Escherichia coli and Pseudomonas aeruginosa in response to PAEs through quorum sensing (QS). These strains degraded over 90% of diethyl phthalate and benzyl butyl phthalate in 12 h, and 80-90% of DEHP in 36-48 h, maintaining high efficiency over 5-round repeating cycles. The combination of protein engineering and QS enables the development of new whole-cell degrader strains with an enhanced degrading capacity and broad substrate tolerance.