文献类型：期刊文献

英文题名：Metabolic Engineering of Pseudomonas putida KT2440 for Complete Mineralization of Methyl Parathion and γ-Hexachlorocyclohexane

作者：Gong, Ting[1,2];Liu, Ruihua[1,2];Zuo, Zhenqiang[1,2];Che, You[1,2];Yu, Huilei[3];Song, Cunjiang[1,2];Yang, Chao[1,2]

机构：[1]Nankai Univ, Minist Educ, Key Lab Mol Microbiol & Technol, Tianjin 300071, Peoples R China;[2]Nankai Univ, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China;[3]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2016

卷号：5

期号：5

起止页码：434

外文期刊名：ACS SYNTHETIC BIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000376476900008)】；

基金：This work was supported by National Key Basic Research Program of China (No. 2012CB725204), National Key Technology Support Program of China (No. 2015BAD16B04), National Natural Science Foundation of China (Nos. 31300032 and 31570035), Project of Tianjin, China (No. 13JCQNJC09700), Open Fund of State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences (No. SKLMR-20130604), and Open Fund of State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology.

语种：英文

外文关键词：methyl parathion; gamma-hexachlorocyclohexane; complete mineralization; Pseudomonas putida KT2440

摘要：Agricultural soils are often cocontaminated with multiple pesticides. Unfortunately, microorganisms isolated from natural environments do not possess the ability to simultaneously degrade different classes of pesticides. Currently, we can use the approaches of synthetic biology to create a strain endowed with various catabolic pathways that do not exist in a natural microorganism. Here, we describe the metabolic engineering of a biosafety Pseudomonas putida strain KT2440 for complete mineralization of methyl parathion (MP) and gamma-hexachlorocyclohexane (gamma-HCH) by functional assembly of the MP and gamma-HCH mineralization pathways. The engineered strain was genetically stable, and no growth inhibition was observed. Such a strain not only would reduce the toxicity of MP and gamma-HCH but also would prevent the accumulation of potentially toxic intermediates in the environment. Furthermore, expression of Vitreoscilla hemoglobin improved the ability of the engineered strain to sequester O-2. The inoculation of the engineered strain to soils treated with MP and gamma-HCH resulted in a higher degradation rate than in noninoculated soils. Moreover, introduced GFP may be used to monitor the activity of the engineered strain during bioremediation. The engineered strain may be a promising candidate for in situ bioremediation of soil cocontaminated with MP and gamma-HCH.