文献类型：期刊文献

中文题名：L-谷氨酸氧化酶产生菌理性化筛选及液体发酵的研究

英文题名：Rational Screening of L-Glutamate Oxidase (GO) Producing Strains and Submerged Fermentation

作者：李青山[1];王立军[1];李友荣[1];谢幸珠[1];李晓波[1]

机构：[1]华东化工学院生化工程研究所

年份：1993

卷号：19

期号：5

起止页码：567

中文期刊名：华东化工学院学报

收录：国家哲学社会科学学术期刊数据库;Scopus;CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：谷氨酸;氧化酶;发酵;筛选

外文关键词：glutamate; glutamate-oxidase; submerged fermentation; rational screening; red spots development

摘要：采用HNO_2诱变、平皿显色与理性化筛选方法,获得一株L-谷氨酸氧化酶高产稳定菌株Streptomyces sp.N_514。建立了液体发酵产酶的条件,考察了辅加成分对酶合成的影响。
Using red spots development and rational screening method, along with mutagenesis, the mutant Streptomyces sp.N_5 14 was obtained, which productivity of GO was 19 folds more than its parent strain sp. 26 and stable after transferring 15 generations. The conditions of GO producing in submerged fermentation were set up and various factors which influence the productivity of GO were studied.