文献类型：期刊文献

英文题名：Dynamics of a Molecular Plug Docked onto a Solid-State Nanopore

作者：Shi, Xin[1];Li, Qiao[1];Gao, Rui[1];Si, Wei[2,3,4,5];Liu, Shao-Chuang[1];Aksimentiev, Aleksei[2,3];Long, Yi-Tao[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[2]Univ Illinois, Dept Phys, 1110 West Green St, Urbana, IL 61801 USA;[3]Univ Illinois, Beckman Inst Adv Sci & Technol, 1110 West Green St, Urbana, IL 61801 USA;[4]Southeast Univ, Jiangsu Key Lab Design & Manufacture Micronano Bi, Nanjing 210096, Jiangsu, Peoples R China;[5]Southeast Univ, Sch Mech Engn, Nanjing 210096, Jiangsu, Peoples R China

年份：2018

卷号：9

期号：16

起止页码：4686

外文期刊名：JOURNAL OF PHYSICAL CHEMISTRY LETTERS

收录：;EI(收录号：20183305681543);WOS:【SCI-EXPANDED(收录号:WOS:000442446900033)】；

基金：We acknowledge Prof. Cees Dekker and his group for valuable discussions. We thank Daniel Verschueren for the discussions, comments, and help during the preparation of the manuscript. We greatly thank Prof. Mark Howarth and his group for providing the monovalent streptavidin. This research was supported by the National Natural Science Foundation of China (21421004, 21327807), the Program of Introducing Talents of Discipline to Universities (B16017), United States National Institutes of Health (R01-HG007406 and P41-GM104601), and China Scholarship Council (CSC201506090040 for W.S. and CSC201606740021 for X.S.).

语种：英文

外文关键词：DNA - Gene encoding - DNA sequences - Proteins

摘要：Docking of a protein DNA complex onto a nanopore can provide ample observation time, and has enabled collection of analytic applications of biological nanopores, including DNA sequencing. However, the application of the same principle to solid-state nanopores is tempered by poor understanding of the docking process. Here, we elucidate the behavior of individual protein DNA complexes docked onto a solid-state nanopore by monitoring the nanopore ionic current. Repeat docking of monovalent streptavidin DNA complexes is found to produce ionic current blockades that fluctuate between discrete levels. We elucidate the roles of the protein plug and the DNA tether in the docking process, finding the docking configurations to determine the multitude of the current blockade levels, whereas the frequency of the current level switching is determined by the interactions between the molecules and the solid-state membrane. Finally, we prove the feasibility of using the nanopore docking principle for single-molecule sensing using solid-state nanopores by detecting conformational changes of a tethered DNA molecule from a random coil to an i-motif state.