文献类型：期刊文献

英文题名：A novel technique for in situ aggregation of Gluconobacter oxydans using bio-adhesive magnetic nanoparticles

作者：Ni, Kefeng[1];Lu, Huimin[1];Wang, Cunxun[1];Black, Kvar C. L.[2,3];Wei, Dongzhi[1];Ren, Yuhong[1];Messersmith, Phillip B.[2,3]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Northwestern Univ, Dept Biomed Engn, Evanston, IL 60208 USA;[3]Northwestern Univ, Chem Life Proc Inst, Evanston, IL 60208 USA

年份：2012

卷号：109

期号：12

起止页码：2970

外文期刊名：BIOTECHNOLOGY AND BIOENGINEERING

收录：;EI(收录号：20124415634684);WOS:【SCI-EXPANDED(收录号:WOS:000310465300009)】；

基金：The authors thank Dr. Jose G. Rivera, Dr. Li-hong He and Dr. Zhongqiang Liu for their useful discussions. This work was funded by The National Natural Foundation of China (No. 21076079), Open Funding Project of the State Key Laboratory of Bioreactor Engineering, the Fundamental Research Funds for the Central Universities, and partially by National Institutes of Health grant R37DE014193. KCLB was supported by a Ruth Kirschstein National Research Service Award (NRSA) from the National Institute of Dental and Craniofacial Research (NIH F31 DE019750).

语种：英文

外文关键词：cell aggregation; iron oxide nanoparticles; Gluconobacter oxydans; polydopamine

摘要：Here, we present a novel technique to immobilize magnetic particles onto whole Gluconobacter oxydans in situ via a synthetic adhesive biomimetic material inspired by the protein glues of marine mussels. Our approach involves simple coating of a cell adherent polydopamine film onto magnetic nanoparticles, followed by conjugation of the polydopamine-coated nanoparticles to G. oxydans which resulted in cell aggregation. After optimization, 21.3 mg (wet cell weight) G. oxydans per milligram of nanoparticle was aggregated and separated with a magnet. Importantly, the G. oxydan aggregates showed high specific activity and good reusability. The facile approach offers the potential advantages of low cost, easy cell separation, low diffusion resistance, and high efficiency. Furthermore, the approach is a convenient platform technique for magnetization of cells in situ by direct mixing of nanoparticles with a cell suspension. Biotechnol. Bioeng. 2012; 109: 29702977. (C) 2012 Wiley Periodicals, Inc.