文献类型：期刊文献

英文题名：Sensitive detection of protein biomarkers using silver nanoparticles enhanced immunofluorescence assay

作者：Zhao, Li-Jun[1,2];Yu, Ru-Jia[1,2];Ma, Wei[1,2];Han, Huan-Xing[3];Tian, He[1,2];Qian, Ruo-Can[1,2];Long, Yi-Tao[1,2]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Key Lab Adv Mat, Shanghai, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, Dept Chem, Shanghai, Peoples R China;[3]Second Mil Med Univ, Changzheng Hosp, Translat Med Ctr, Shanghai, Peoples R China

年份：2017

卷号：7

期号：4

起止页码：876

外文期刊名：THERANOSTICS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000396559600006)】；

基金：This research was supported by the 973 Program (Grants 2013CB733700), the National Natural Science Foundation of China (No. 21327807, 21421004), and the Program of Shanghai Subject Chief Scientist (No. 15XD1501200), the Programme of Introducing Talents of Discipline to Universities (No. B16017).

语种：英文

外文关键词：silver nanoparticles; magnetic beads; fluorescence detection; biomarkers

摘要：Detection of biomarkers is extremely important for the early diagnosis of diseases. Here, we developed an easy and highly sensitive fluorescence detection system for the determination of biomarkers by combining the rapid separation of magnetic beads and silver nanoparticles labeled antibodies. An ultrasensitive silver ions fluorescence probe 3', 6'-bis (diethylamino)-2-(2-iodoethyl) spiro[ isoindoline-1, 9'-xanthen]-3-one (Ag+-FP) was applied to immunoassay. A significant signal amplification was achieved as the AgNPs can be dissolved by H2O2 and generate numerous Ag+, which would turn "on" the fluorescence of Ag+-FP. Using a-fetoprotein (AFP) and C-reactive protein (CRP) as target analytes, good linear responses were obtained from 0.1 to 10 ng mL(-1) and the limits of detection (LOD) were as low as 70 pg.mL(-1) and 30 pg.mL(-1), respectively. In addition, the developed system was further evaluated for the detection of real samples including 30 positive serum specimens obtained from hepatocarcinoma patients and 20 negative serum samples, and performs as well as the commercial electrochemiluminescence immunoassay (ECLI) method with less cost and more convenience. Thus, the designed detection system can be used as a promising platform for the detection of a variety of biomarkers and served as a powerful tool in clinical diagnosis.