文献类型：期刊文献

中文题名：丙氨酸和三甲胺对林可霉素发酵的影响及部分相关基因的转录水平

英文题名：Lincomycin fermentation in response to alanine and trimethylamine application and transcription level of related gene

作者：张海丹[1];叶蕊芳[1];毛全贵[2];吕和平[2];刘学威[2];冯汇[2]

机构：[1]华东理工大学生物工程学院,上海200237;[2]河南天方药业有限公司,河南驻马店463600

年份：2014

卷号：30

期号：5

起止页码：1003

中文期刊名：江苏农业学报

外文期刊名：Jiangsu Journal of Agricultural Sciences

收录：CSTPCD;;北大核心:【北大核心2011】；CSCD:【CSCD_E2013_2014】；

语种：中文

中文关键词：林可霉素;丙氨酸;三甲胺;丙酮酸激酶;邻氨基苯甲酸合成酶;lmbG基因

外文关键词：lincomycin;alanine;trimethylamine;pyruvate kinase;anthranilate synthase;lmbG gene

摘要：为了研究添加丙氨酸和三甲胺对林可霉素工业生产菌株Streptomyce lincolnensis发酵过程中的工艺参数和相关基因转录的影响,对发酵液和菌体分别进行生物效价测定以及lmbG、PK和ASA基因的表达量测定。结果表明:15 L罐发酵水平,在发酵培养24 h时添加0.1 g/L的丙氨酸处理与对照相比效价提高了23%,林肯链霉菌生物合成基因簇中的基因lmbG的转录水平远高于对照,丙酮酸激酶(PK)的基因表达量明显降低;在发酵培养24 h时添加0.02%的三甲胺处理与对照相比效价提高了30%,lmbG的转录水平明显提高,邻氨基苯甲酸合成酶(ASA)的基因转录水平降低。说明添加丙氨酸和三甲胺可以明显提高林可霉素产量,且对其生物合成基因可能具有正向调控作用。
The study mainly focused on the influence on Streptomyces lincolnensis fermentation and related gene tran-scription level by adding alanine or trimethylamine. The fermentation broth and the thalli were measured by biological po-tency and the transcription levels of gene lmbG and genes encoding pyruvate kinase （ PK） and anthranilate synthase（ ASA） were identified. 15-L tank fermentation broth of S. lincolnensis, the potency of lincomycin was increased by 23% by adding 0. 1 g/L alanine at 24-h fermentation, the transcription level of gene lmbG of the S. lincolnensis biosynthesis gene cluster was elevated, and the expression level of the gene encoding PK decreased significantly. Adding 0. 02% trimethylamine in-creased, the yield of lincomycin by 30% and gene lmbG expression, however, the expression of the gene encoding ASA dropped. Adding alanine or trimethylamine during S. lincolnensis fermentation can increase lincomycin production, and＆amp;nbsp;may play a positive role in lincomycin biosynthesis gene regulation.