文献类型：期刊文献

英文题名：BODIPY-based colorimetric/ratiometric fluorescence probes for sulfite in aqueous solution and in living cells

作者：Liu, Shanshan[1];Song, Lun[1];Sun, Qian[1];Chen, Zhaoyang[1];Ge, Yu[2];Zhang, Weibing[1];Qian, Junhong[1]

机构：[1]E China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]Shanghai Inst Qual Inspect & Tech, Shanghai 200233, Peoples R China

年份：2015

卷号：5

期号：111

起止页码：91863

外文期刊名：RSC ADVANCES

收录：;EI(收录号：20154501521394);WOS:【SCI-EXPANDED(收录号:WOS:000364057000093)】；

基金：This work was financially supported by National Natural Science Foundation of China (No. 21576085) and Shanghai Municipal Natural Science Foundation (15ZR1409000).

语种：英文

外文关键词：Fluorophores - Color - Colorimetry - Probes - Absorption spectroscopy - Ketones - Solutions - Addition reactions - Water absorption

摘要：Two colorimetric/ratiometric fluorescence probes (BSP1 and BSP2) for sulfite utilizing the Michael-addition mechanism were designed and synthesized by incorporating a substituted pyridinium/methyl pyridinium to a BODIPY fluorophore linked with an alpha, beta-unsaturated ketone. BSP1 exhibited colorimetric and ratiometric fluorescence responses toward sulfite in PBS-CTAB system. The addition of sulfite to the C=C group in BSP1 induced about 60 nm hypsochromic shift in both absorption (from 554 nm to 494 nm) and emission (from 618 nm to 554 nm) spectra accompanied with fluorescent color change from red to green under hand-lamp excited at 365 nm. Good linear relationship was found between the emission ratio (I-554/I-618) and the concentration of sulfite in the range of 0-300 mu M with a detection limit of 6.4 mM. In the case of BSP2, the probe showed excellent water solubility and faster spectral response to sulfite with about 100 nm blue shift (from 567 nm to 470 nm) in the absorption spectrum in PBS. The solution's color changed from purple to yellow. Both BSP1 and BSP2 displayed high selectivity and competition towards sulfite over other relative species such as thiols and sulfide. More importantly, we have demonstrated that BSP1 could be applied in the imaging of sulfite in living cells.