文献类型：期刊文献

英文题名：Detection on emamectin benzoate-induced apoptosis and DNA damage in Spodoptera frugiperda Sf-9 cell line

作者：Wu, Xiwei[1];Zhang, Lei[1];Yang, Chao[1];Zong, Mimi[1];Huang, Qingchun[1];Tao, Liming[1]

机构：[1]E China Univ Sci & Technol, Shanghai Key Lab Chem Biol, Sch Pharm, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2016

卷号：126

起止页码：6

外文期刊名：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000368966700002)】；

基金：This work was financially supported by the National Key Technology R&D Program (No. 2011BAE06B04) and the Shanghai Foundation of Science and Technology (No. 15431906800). The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.

语种：英文

外文关键词：Emamectin benzoate; Sf-9 cells; Cytotoxicity; DNA damage; Apoptosis

摘要：Emamectin benzoate (EMB), an important macrocyclic lactone insecticide that belongs to the avermectin family and possesses excellent potency in controlling pests, is non-carcinogenic and non-mutagenic conducted in rats and mice, but EMB-induced cytotoxicity and genotoxicity in arthropod insect have been seldom reported yet. In the present paper, we quantified the cytotoxicity of EMB through the detections on cell viability, DNA damage, and cell apoptosis in Spodoptera frugiperda Sf-9 cells in vitro. The results showed that EMB caused a concentration- and time-dependent reduction on the viability of Sf-9 cells, and the median inhibitory concentrations (IC50) were 334 mu M at 72 h of exposure. The dual acridine orange/ethidium bromide staining showed that exposure to EMB induced a significant time- and concentration-dependent increase on cell apoptosis. The alkaline comet assay revealed that EMB induced significant increases on single-strand DNA breaks, and the percentage of gamma H2AX-positive cells represented a time- and concentration-dependent formation of DNA double-strand breaks in Sf-9 cells. Interestingly, the similar cytotoxic actions of EMB also went for the human cancerous Hela cells as a control cell group. Data demonstrated the potential cytotoxic effect of EMB on Sf-9 cells that was significantly greater than the effect of hydrogen peroxide at the same concentrations. (C) 2015 Elsevier Inc. All rights reserved.