文献类型：期刊文献

英文题名：Exploring cellular fatty acid composition and intracellular metabolites of osmotic-tolerant mutant Lactobacillus paracasei NCBIO-M2 for highly efficient lactic acid production with high initial glucose concentration

作者：Tian, Xiwei[1,2];Wang, Yonghong[1,2,3];Chu, Ju[1,2,3];Mohsin, Ali[1,2];Zhuang, Yingping[1,2,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Natl Ctr Bio Engn & Technol Shanghai, Shanghai 200237, Peoples R China;[3]Shanghai Collaborat Innovat Ctr Biomfg, Shanghai 200237, Peoples R China

年份：2018

卷号：286

起止页码：27

外文期刊名：JOURNAL OF BIOTECHNOLOGY

收录：;EI(收录号：20183805845258);WOS:【SCI-EXPANDED(收录号:WOS:000448015600005)】；

基金：This work was financially supported by the National Science Foundation for Young Scientists of China (31700038), the National Key Research and Development Program (2017YFB0309302), the Fundamental Research Funds for the Central Universities (WF1814032, 22221817014, 22221818014).

语种：英文

外文关键词：Lactobacillus paracasei; Lactic acid; Osmotic pressure; Fatty acid; Intracellular metabolite

摘要：High titer, productivity and yield are the main pre-requisites of an efficient lactic acid production process. However, the hyperosmotic stress inhibits cell metabolism in the later phase of fermentation. In this study, an osmotic-tolerant mutant named Lactobacillus paracasei NCBI001-M2 was obtained through a high-throughput screening technology, which exhibited a higher tolerance to osmotic stress due to its more flexible regulation of the unsaturated fatty acid proportion along with the intracellular compatible solute pools. The mutant successfully consumed all 248 g/L initial glucose and produced 223.7 g/L lactic acid with a productivity of 5.53 g/ L/h in a single batch fermentation by the neutralizing agent strategy. Moreover, similar fermentation performances were also achieved in the open fermentation mode without sterilization by the mutant, which suggested that the mutant would be a potential for cost-effective commercial lactic acid production.