文献类型：期刊文献

英文题名：Rational High-Throughput System for Screening Emodin High-Yielding Mutant from Marine Strains of Aspergillus flavipes HN4-13

作者：Gong, Lizhi[1];Li, Zixuan[1];Xu, Meina[2];Zhou, Yushan[1];Zhang, Wenqing[1];Zhao, Jian[1];Xin, Xiujuan[1];An, Faliang[1,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Mei Long Rd, Shanghai 200237, Peoples R China;[2]Jinan Univ, Coll Pharm, Guangzhou 510632, Peoples R China;[3]Marine Biomed Sci & Technol Innovat Platform Lin g, 4 Lane 218,Haiji Sixth Rd, Shanghai 201306, Peoples R China

年份：2025

卷号：23

期号：4

外文期刊名：MARINE DRUGS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001475508300001)】；

基金：This research was supported by the National Key R&D Program of China (2018YFC1706206, 2019YFC0312504) and the National Natural Science Foundation of China (41876189, 81703388). This work was also supported by the Open Research Fund Program of State Key Laboratory of Bioreactor Engineering and Shanghai Collaborative Innovation Center for Biomanufacturing Technology.

语种：英文

外文关键词：marine strain Aspergillus flavipes HN4-13; emodin; ARTP; high-throughput screening; medium optimize

摘要：Emodin is an anthraquinone compound known for its diverse biological activities, including anti-tumor and anti-inflammatory effects, making it highly applicable in the fields of biology and medicine. The production of emodin using microorganisms represents a sustainable and environmentally friendly approach. A marine-derived Aspergillus flavipes HN4-13 was found to produce emodin, but the yield was too low for industrial production. To develop a high-yielding emodin-producing strain, we established the high-through detection and screening methods of alkaline coloration and deep-well plant culture, enabling the effective selection of high-yielding strains. Following ARTP mutagenesis of the wild strain A. flavipes HN4-13, the resulting mutant strain, M1440, exhibited an increased emodin yield of 124.6 +/- 4.95 mg/L. Furthermore, the production of the emodin was enhanced by the exogenous addition of metal ions Mn2+ to the medium. Specifically, the addition of 3 mM Mn2+ resulted in a 133.2% increase in emodin production, with the highest yield reaching 178.6 +/- 7.80 mg/L.